So I performed BCA assay to determine how much amount of protein is immobilized on my particle. And I got the concentration of for eg. 0.44mg/ml. And the initial amount I added was 8mg (4ml of 2mg/ml). Now I am referring to many paper and there are many terminology that has been used (immobilization efficiency, loading efficiency, immobilization yield, etc .) I used this procedure: enzyme immobilized particle was incubate in buffer for 30 mins and 25 microliter of supernatant was used for BSA assay. Does anyone have an idea how can I determine how much amount of protein is immobilized on my p ..read more

I work in a hospital setting and have spare Beta-CrossLaps test kits expiring in November of this year. My supervisor has asked me to collaborate with an endocrinologist (which I can skip ig) and conduct research using these kits. Could anyone please provide guidance or suggestions for potential research ideas? submitted by /u/woodenslabs [visit reddit] [comments ..read more

I got the opportunity to study biochemistry at a college tuition free, but my parents think it’s a better idea to study nursing at a slightly more expensive, smaller college. I have no issue studying either and even have a slight preference for nursing because my preferred workplace is a hospital/clinic, but I’d prefer to go to a college that’s better financially for my parents and allows me to be somewhat financially independent from them, plus it’s in a better location. Keep in mind, my parents have no issue paying for the more expensive college as I got a scholarship there too so it’s rela ..read more

Hi there, I am currently graduating from a BSc in biochem (minor in chemistry) and I am wondering if i can use this to become a pharmacist. Should there be enough overlap in my degree and a PharmD program to make it worth it? I am hoping to use my bachelors rather than do another 4 years of undergrad. For reference I live in Canada (NL) submitted by /u/ragufriend [visit reddit] [comments ..read more

Gday fellow biochemists! I’m a biochemist who changed into Bioinformatic sciences. Currently learning structural bioinformatics at my uni. While reviewing very basic (!) protein structures (peptide synthesis), I was exposed to a paper in nature here, 2021 which talks about NOS bridges as covalent bridges next to disulfide bridges. What’s the state of the art here now a few years later? Didn’t find any great reception to be honest, maybe I’m wrong. submitted by /u/Toqra1 [visit reddit] [comments ..read more

The last few days i havent been able to open massbank EU and it shows 'proxy error'. Does anyone know an alternative for massbank? submitted by /u/renskeeeee [visit reddit] [comments ..read more

Research and medical I have two options right now. What would be the best considering my interest in research ( basic and translation. Not much keen about clinical research) 1) pursue bsc in bio-chemistry, followed by master's and PhD abroad in area of interest. ( Assuming I am able to get a good college abroad) 2) Do mbbs then pursue PhD ( or can I do research without a PhD ? That is with a mbbs and MD ? In the USA it is medschool + phd. Concerned about : A} If medical knowledge would be useful for basic/translation research? ( Not considering clinical research) B} would a med student be pre ..read more

Hi! I'm a senior in high school and I'm going to be majoring in biochemistry this fall. I was very split between bio, bioe, and biochem. To be honest, I chose it because I thought I liked chemistry more than biology, but I'm not sure if that's because if I did better in it, if my good teachers made the subject more enjoyable, or if it's because I actually enjoy the subject. I haven't been as good at biology -- my teacher's teaching methods have not worked on me, but I do like some of the concepts. For some additional context: I enjoyed my introductory chemistry class much more than my AP chem ..read more

In the college I will be attending in the fall as a Biochemistry major, physics and calculus are not required for my degree. The only sciences I am required to take are chemistry’s and biology’s (besides General Chemistry I & II due to me having a sufficient score on the AP Chemistry exam) and the only math I have to take is statistics, which I’ve already completed in high school through means of dual enrollment credit that my college will be accepting. However, almost every college where I look/have looked, they require physics and/or calculus for their degrees. My question is: is me not ..read more

Just looking for something interesting to read. I recently got my BS in Biochemistry at UCLA but I have a little baby and I am currently staying home/not working but my brain is itching for something interesting to read. Thank you. I like topics in yeast, scared of prions but fascinated by it, human disease/advancements, food/nutrition. Really anything that I can print and read (I have access to most papers through my school's library). submitted by /u/PitchPrior7655 [visit reddit] [comments ..read more