Facts and information about Bioinformatics especially for DBT-JRF:  [Part -2] These notes are only for the students who are not interested in Bioinformatics but want to solve and memorize the common questions in DBT-JRF.  These are just the random facts, prepared from analyzed PYQs. The following are handwritten notes. If you face any problems please let us know in the comment section.  ..read more

 Facts and information about Bioinformatics especially for DBT-JRF:  [Part -1] These notes are only for the students who are not interested in Bioinformatics but want to solve and memorize the common questions in DBT-JRF.   DOT BLOT: Pairwise sequence similarity   Protein database: Try to recall the names and the subcategories.   E- value:  BLAST: Have a look at the difference between local and global alignment.  If a nucleotide sequence encoding a protein is known and a homologous protein to be identified, --> the best analysis tool is B ..read more

 The following notes are the handwritten notes for Life science competitive exams (mainly SET). It is helpful for UGC NET too.  If you face any kind of problems please let us know in the comment section.  ..read more

 Crabtree effect: The name is given by an English Biochemist Herbert Grace Crabtree.  This effect is referred to as the production of ethanol in aerobic conditions rather than biomass by yeast cells at high external glucose concentrations through the TCA cycle.  This is basically the decrease in the rate of electron transport due to the mass action of the elevated phosphorylation ratio.  Warburg effect: This effect is referred to as an increase in the rate of glucose uptake and the production of lactate in aerobic conditions. The discoverer of this effect,  Warburg ..read more

 The following suggestions for Chemistry numerical (HS-2024- Bengali version) are uploaded. If any kind of problems arise, please let us know in the comment section.  All the best ..read more

The following suggestions for Biology (HS-2024- Bengali version) are hand-written. If any kind of problems arise, please let us know in the comment section.   All the best ..read more

  MTT Assay Principle: The MTT assay provides a readout of cell viability & growth that works by measuring cell metabolic activity. The yellow tetrazolium MTT (3-(4, 5-dimethyl thiazolyl-2)-2, 5-diphenyltetrazolium bromide) is reduced by the metabolically active cells, by the action of dehydrogenase enzymes, to produce reducing equivalents such as NADH and NADPH. The resulting intracellular purple formazan can be solubilized and quantified by spectrophotometric means at 570nm. MTT is sensitive to light. This assay should be performed in the dark. The MTT assay measures, cellular ..read more

The Basic steps of the cell-reviving process  1)  Cryopreserved Cells [with DMSO (Dimethyl sulfoxide)] were collected from the -80°C. 2) Then the cells were thawed rapidly in the water bath at 37°C for 30-45 sec to protect the cells from DMSO. 3) The cells were transferred into a 15 ml falcon which already contains 6 ml of media [DMEM (Dulbecco’s Modified Eagle Medium) + 10% FBS (Foetal Bovine Serum) + 1X penicillin-streptomycin solution]. 4) The centrifugation was performed at 1100 rpm for 5 minutes. 5) The Supernatant was discarded in the beaker con ..read more

  Keto diet: How does this diet work: The establishment of the keto diet is if glucose (the simplest form of carbohydrate as well as the main source of energy in every animal) is deprived, which is gained by consuming carbs. The ketones are a substitution of glucose, which is synthesized from stored fat. Hence, the name is ketogenic. The neurocytes (The brain cells) need glucose approximately 120 grams per day because these cells cannot store glucose. When a person is fasting or consumes very little carbohydrates, at first the body pulls glucose from the liver and muscle glycogen. Glycoge ..read more

 Cyanide in Apple!!? Apple ?seeds are often regarded as a healthy snack that is packed with numerous nutritional benefits. However, there is a concern that apple seeds contain cyanide, a toxic substance that can cause harm to the human body. In this article, we will discuss the truth behind apple seeds containing cyanide, how much cyanide is present, and whether eating apple seeds is safe or not. The truth behind apple seeds containing cyanide Apple seeds contain a small amount of cyanide, which is a poisonous chemical compound that can be harmful to the body if ingested in large amoun ..read more