Suppose my body is invaded by bacteria and this bacteria multiplies quickly, has a short life span, white blood cells do not phagocytize all, and dead bacteria that have expired are located everywhere in the body. The question is that dead bacteria Is it trapped in the ECM (extracellular matrix)?, if too much will it cause type 2 hypersensitivity? submitted by /u/Alternative-Sun-4449 [visit reddit] [comments ..read more

Mouse T cells are more sensitive than Human T cells, are IL2 concentration play a significant role in T cell activation via CD3/CD28? Would too much IL2 kill my mouse T cell invitro culture? submitted by /u/JacksonSxcc [visit reddit] [comments ..read more

Location : USA, EU, AUS submitted by /u/notcallipygian [visit reddit] [comments ..read more

For primary cell culture I have to combine cells from multiple mice to increase the cell numbers. I know you can pool the cells of mice with the same genotype, but would you also pool cells from male and female mice with the same genotype? For primary microglia cultured from newborn mice we do pool multiple brains (sex is not known because of the age p1-4). I am also doing primary cell culture using adult mice, so the sex is known, but it is sometimes difficult to get enough animals of the same sex and age since the mouse line is not very fertile. Can I then combine female and male cells for ..read more

submitted by /u/Mobilityqueen516 [visit reddit] [comments ..read more

Why do some of them have long strings of the letter b? Why are they not given more descriptive names? Why does it feel almost random? Why am I so angry about this? Biology is full of slightly strange naming conventions, which I understand. Certain proteins are discovered in one context but are found in others after their discovery e.g BNP, TNF alpha. But these are forgivable, but why on earth would you call something so robotic. This post isn’t calling for an upheaval of the naming system, which would ultimately create more confusion as people would find different names for the same thing whe ..read more

Interesting paper I found and a (probably terrible) idea from a biology student https://www.science.org/doi/10.1126/scitranslmed.adi1145?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed Epitope base editing of CD45 to create a modified version that is not recognized and attacked by CD-45 targeting CAR-T cells. Scientists testing it in pre-clinical studies for use against leukemia, where CD-45 targeting CAR-T cells (which have the modified CD-45 antigen as well to prevent them from killing each other) kill the leukemia as well as all healthy white blood cells ..read more

Hi all, I was hoping someone with some experience doing T cell activation induced marker assays could shed some light on my situation. I want to do T cell activation based cell sorting of PBMCs based on expression of activation markers following a 24 hour peptide pool stimulation. Typically, this is done with 1 million cells per well of a 96-well and the final concentration of the peptides is 1ug/mL. Everywhere I look in the literature it is done this way, never more than 1M cells per well. Well, I want to scale this up to 5M or more cells. I tried this once w 5M cells in a 96 well and the me ..read more

submitted by /u/Fair-Plantain-8851 [visit reddit] [comments ..read more

I am new to this subreddit, so direct me to the right subreddit if needed. I am writing an undergraduate research paper on the potentially harmful effects of monoclonal antibodies used for organ transplant patients. I need help finding resources on this. I understand monoclonal antibodies are somewhat new as a treatment option, so maybe there isn't enough data. I would appreciate any insight on this subject or papers I can read. submitted by /u/johannes_grun [visit reddit] [comments ..read more