Nature Cell Biology, Published online: 19 April 2024; doi:10.1038/s41556-024-01407-w Reicher, Reiniš et al. report a method for multicolour tagging using genome-scale intron-targeting sgRNA libraries that, in combination with computer vision, enables the systematic detection of protein localization changes ..read more

Nature Cell Biology, Published online: 19 April 2024; doi:10.1038/s41556-024-01404-z Our understanding of the basic mechanisms of autophagy is growing, but many questions remain about the types of autophagy cells use, when they use them, and how they function in different contexts. We asked emerging and established leaders in the field to discuss the questions and areas that they are most excited about to deepen our understanding of autophagy ..read more

Nature Cell Biology, Published online: 12 April 2024; doi:10.1038/s41556-024-01381-3 Volume electron microscopy (vEM) generates large 3D volumes of cells or tissues at nanoscale resolutions, enabling analyses of organelles in their cellular environment. Here, we provide examples of vEM in cell biology and discuss community efforts to develop standards in sample preparation and image acquisition for enhanced reproducibility and data reuse ..read more

Nature Cell Biology, Published online: 11 April 2024; doi:10.1038/s41556-024-01402-1 Chidley et al. report a CRISPR interference/activation screening platform to systematically interrogate the contribution of nutrient transporters to support cancer cell proliferation in environments of variable composition ..read more

Nature Cell Biology, Published online: 10 April 2024; doi:10.1038/s41556-024-01391-1 Three studies identify a transcription-coupled DNA–protein cross-link repair pathway that depends on the Cockayne syndrome proteins and the proteasome ..read more

Nature Cell Biology, Published online: 10 April 2024; doi:10.1038/s41556-024-01401-2 Three studies identify a transcription-coupled DNA–protein crosslink repair pathway that depends on the Cockayne syndrome proteins and the proteasome ..read more

Nature Cell Biology, Published online: 10 April 2024; doi:10.1038/s41556-024-01394-y Three studies identify a transcription-coupled DNA–protein crosslink repair pathway that depends on the Cockayne syndrome proteins and the proteasome ..read more

Nature Cell Biology, Published online: 10 April 2024; doi:10.1038/s41556-024-01392-0 Organ morphogenesis begins with proliferation, which results in tissue pressures and site-specific YAP expression, nuclear translocation and signalling. A study now reports the involvement of anisotropy, localized pressure and YAP signalling in organizer-forming cascades, introducing a new chapter of molecular mechanobiology of organogenesis ..read more

Nature Cell Biology, Published online: 10 April 2024; doi:10.1038/s41556-024-01399-7 When transcription by RNA polymerase II is stalled by ultraviolet-induced DNA damage, it recruits repair factors, leading to excision of the damaged site and DNA synthesis to fill the gap. Three new studies show that, for aldehyde-induced DNA crosslinks, repair is activated by the same factors, but without base excision and gap filling ..read more

Nature Cell Biology, Published online: 09 April 2024; doi:10.1038/s41556-024-01403-0 Scarfò, Randolph et al. perform transcriptomic analysis of 28- to 32-day human embryos and identify CD32 as a marker of haemogenic endothelial cells (HECs), thus providing a strategy to isolate HECs from human embryos and pluripotent stem cell cultures ..read more