Nature Cell Biology, Published online: 12 April 2024; doi:10.1038/s41556-024-01381-3 Volume electron microscopy (vEM) generates large 3D volumes of cells or tissues at nanoscale resolutions, enabling analyses of organelles in their cellular environment. Here, we provide examples of vEM in cell biology and discuss community efforts to develop standards in sample preparation and image acquisition for enhanced reproducibility and data reuse ..read more

Nature Cell Biology, Published online: 11 April 2024; doi:10.1038/s41556-024-01402-1 Chidley et al. report a CRISPR interference/activation screening platform to systematically interrogate the contribution of nutrient transporters to support cancer cell proliferation in environments of variable composition ..read more

Nature Cell Biology, Published online: 10 April 2024; doi:10.1038/s41556-024-01391-1 Three studies identify a transcription-coupled DNA–protein cross-link repair pathway that depends on the Cockayne syndrome proteins and the proteasome ..read more

Nature Cell Biology, Published online: 10 April 2024; doi:10.1038/s41556-024-01401-2 Three studies identify a transcription-coupled DNA–protein crosslink repair pathway that depends on the Cockayne syndrome proteins and the proteasome ..read more

Nature Cell Biology, Published online: 10 April 2024; doi:10.1038/s41556-024-01394-y Three studies identify a transcription-coupled DNA–protein crosslink repair pathway that depends on the Cockayne syndrome proteins and the proteasome ..read more

Nature Cell Biology, Published online: 10 April 2024; doi:10.1038/s41556-024-01392-0 Organ morphogenesis begins with proliferation, which results in tissue pressures and site-specific YAP expression, nuclear translocation and signalling. A study now reports the involvement of anisotropy, localized pressure and YAP signalling in organizer-forming cascades, introducing a new chapter of molecular mechanobiology of organogenesis ..read more

Nature Cell Biology, Published online: 10 April 2024; doi:10.1038/s41556-024-01399-7 When transcription by RNA polymerase II is stalled by ultraviolet-induced DNA damage, it recruits repair factors, leading to excision of the damaged site and DNA synthesis to fill the gap. Three new studies show that, for aldehyde-induced DNA crosslinks, repair is activated by the same factors, but without base excision and gap filling ..read more

Nature Cell Biology, Published online: 09 April 2024; doi:10.1038/s41556-024-01403-0 Scarfò, Randolph et al. perform transcriptomic analysis of 28- to 32-day human embryos and identify CD32 as a marker of haemogenic endothelial cells (HECs), thus providing a strategy to isolate HECs from human embryos and pluripotent stem cell cultures ..read more

Nature Cell Biology, Published online: 09 April 2024; doi:10.1038/s41556-024-01400-3 Liu et al. find that long-chain acyl-coenzyme A activates two mitochondrial fission proteins, MiD49 and MiD51, by inducing their oligomerization. This activates their ability to stimulate DRP1 GTPase activity and triggers mitochondrial division ..read more

Nature Cell Biology, Published online: 08 April 2024; doi:10.1038/s41556-024-01389-9 Ugolini et al. show that transcription bodies regulate gene expression during zygotic genome activation in zebrafish development by sequestering CDK9 to limit the transcription of genes away from transcription bodies ..read more