Precise in vivo RNA base editing with a wobble-enhanced circular CLUSTER guide RNA
Nature » Biotechnology
by Philipp Reautschnig, Carolin Fruhner, Nicolai Wahn, Charlotte P. Wiegand, Sabrina Kragness, John F. Yung, Daniel T. Hofacker, Jenna Fisk, Michelle Eidelman, Nils Waffenschmidt, Maximilian Feige, Laura S. Pfeiffer, Annika E. Schulz, Yvonne Füll, Erez Y. Levanon, Gail Mandel, Thorsten Stafforst
22h ago
Nature Biotechnology, Published online: 12 July 2024; doi:10.1038/s41587-024-02313-0 Using G•U wobble base pairs at specific loci increases RNA base-editing precision and efficiency ..read more
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Template-independent enzymatic synthesis of RNA oligonucleotides
Nature » Biotechnology
by Daniel J. Wiegand, Jonathan Rittichier, Ella Meyer, Howon Lee, Nicholas J. Conway, Daniel Ahlstedt, Zeynep Yurtsever, Dominic Rainone, Erkin Kuru, George M. Church
22h ago
Nature Biotechnology, Published online: 12 July 2024; doi:10.1038/s41587-024-02244-w Single-stranded RNA is enzymatically synthesized without use of a template strand ..read more
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Enzymatic synthesis of RNA oligonucleotides
Nature » Biotechnology
by Marcel Hollenstein
22h ago
Nature Biotechnology, Published online: 12 July 2024; doi:10.1038/s41587-024-02322-z Research on enzymatic RNA synthesis has long been eclipsed by work on DNA—but a new method provides a leap forward for RNA ..read more
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G•U wobble base pairs improve site-directed RNA base editing
Nature » Biotechnology
by
22h ago
Nature Biotechnology, Published online: 12 July 2024; doi:10.1038/s41587-024-02323-y Improving the precision of gene correction through RNA base editing typically involves a trade-off with efficiency. We find using G•U wobble base pairs is a universally applicable strategy to improve editing precision and efficiency, and is effective in a mouse model of Rett syndrome in vivo ..read more
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CAR-Ts sweep into autoimmunity
Nature » Biotechnology
by Charlotte Harrison
4d ago
Nature Biotechnology, Published online: 10 July 2024; doi:10.1038/s41587-024-02321-0 Biotechs are equipping CAR-T cells to destroy B cells — not to treat blood cancers, but to take on multiple sclerosis and a raft of autoimmune disorders ..read more
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Complete substitution with modified nucleotides in self-amplifying RNA suppresses the interferon response and increases potency
Nature » Biotechnology
by Joshua E. McGee, Jack R. Kirsch, Devin Kenney, Faith Cerbo, Elizabeth C. Chavez, Ting-Yu Shih, Florian Douam, Wilson W. Wong, Mark W. Grinstaff
6d ago
Nature Biotechnology, Published online: 08 July 2024; doi:10.1038/s41587-024-02306-z Complete 5-methylcytidine substitution enhances the efficacy of self-amplifying RNA ..read more
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Breathing new life into in vivo lung editing
Nature » Biotechnology
by
6d ago
Nature Biotechnology, Published online: 08 July 2024; doi:10.1038/s41587-024-02338-5 New lipid nanoparticle designs make targeted in vivo lung editing possible in mice and may lead to new treatment options for cystic fibrosis ..read more
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Editor’s pick: Unnatural Products
Nature » Biotechnology
by Michael Eisenstein
1w ago
Nature Biotechnology, Published online: 03 July 2024; doi:10.1038/s41587-024-02286-0 Each year, Nature Biotechnology highlights companies that have received sizeable early-stage funding in the previous year. Unnatural Products is rewriting the rules for potent oral therapies ..read more
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Tracking-seq reveals the heterogeneity of off-target effects in CRISPR–Cas9-mediated genome editing
Nature » Biotechnology
by Ming Zhu, Runda Xu, Junsong Yuan, Jiacheng Wang, Xiaoyu Ren, Tingting Cong, Yaxian You, Anji Ju, Longchen Xu, Huimin Wang, Peiyuan Zheng, Huiying Tao, Chunhua Lin, Honghao Yu, Juanjuan Du, Xin Lin, Wei Xie, Yinqing Li, Xun Lan
1w ago
Nature Biotechnology, Published online: 02 July 2024; doi:10.1038/s41587-024-02307-y The off-target effects of any CRISPR-based genome editor are identified with Tracking-seq ..read more
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High-throughput discovery of MHC class I- and II-restricted T cell epitopes using synthetic cellular circuits
Nature » Biotechnology
by Ayano C. Kohlgruber, Mohammad H. Dezfulian, Brandon M. Sie, Charlotte I. Wang, Tomasz Kula, Uri Laserson, H. Benjamin Larman, Stephen J. Elledge
1w ago
Nature Biotechnology, Published online: 02 July 2024; doi:10.1038/s41587-024-02248-6 T cell-specific antigens are discovered at high throughput without killing target cells ..read more
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