Nature Chemical Biology, Published online: 15 May 2023; doi:10.1038/s41589-023-01338-x A combination of enzyme discovery for nonheme diiron N-monooxygenases, metabolic engineering and genetic code expansion enables the construction of a live bacterial producer of synthetic nitrated proteins containing para-nitro-l-phenylalanine ..read more

Nature Chemical Biology, Published online: 15 May 2023; doi:10.1038/s41589-023-01319-0 Identification of the molecular features that define the kinetic stability of the clinically relevant NDM-1 metallo-β-lactamase in the bacterial periplasm links the cellular metabolism of this protein with its natural evolution ..read more

Nature Chemical Biology, Published online: 15 May 2023; doi:10.1038/s41589-023-01327-0 The medicinal plant Catharanthus roseus is a source of leading anticancer drugs. The monoterpene indole alkaloid (MIA) biosynthetic pathway in C. roseus has now been analyzed using a complementary, multi-omics, single-cell approach. This identified clusters of genes involved in MIA biosynthesis and cell-type-specific partitioning in the MIA biosynthetic pathway ..read more

Nature Chemical Biology, Published online: 15 May 2023; doi:10.1038/s41589-023-01322-5 Protein stability is important for biological function, but little is known about in-cell stability. In the New Delhi metallo-β-lactamase NDM-1, enhancement of zinc binding or amino acid substitutions at the C terminus increase in-cell kinetic stability and prevent proteolysis. These findings link NDM-1-mediated resistance with its in-cell stability and physiology ..read more

Nature Chemical Biology, Published online: 11 May 2023; doi:10.1038/s41589-023-01326-1 A selective inhibitor of Sec61 blocks protein entry into the secretory pathway and has therapeutic efficacy in rheumatoid arthritis. A cryo-EM structure of the inhibited Sec61 provides a model for client-selective protein translocation inhibition ..read more

Nature Chemical Biology, Published online: 11 May 2023; doi:10.1038/s41589-023-01337-y Itskanov and Wang et al. determined high-resolution structures of the human Sec61 channel inhibited by several structurally distinct small molecules and revealed the common inhibitor-binding site in Sec61 and molecular interactions in atomic detail ..read more

Nature Chemical Biology, Published online: 11 May 2023; doi:10.1038/s41589-023-01339-w Cryo-EM structures of complement receptors C3aR and C5aR1 bound to their respective anaphylatoxin ligands C3a and C5a reveal insights into the conserved features and topological diversities of C3aR and C5aR1 in recognizing C3a and C5a ..read more

Nature Chemical Biology, Published online: 11 May 2023; doi:10.1038/s41589-023-01352-z Author Correction: Ancient defensive terpene biosynthetic gene clusters in the soft corals ..read more

Nature Chemical Biology, Published online: 24 April 2023; doi:10.1038/s41589-023-01314-5 Characterization of the conformation landscape of the bacterial ABC transporter CydDC reveals that this heterodimeric protein complex is a long-sought heme transporter essential for the functional maturation of bd-type cytochromes ..read more

Nature Chemical Biology, Published online: 24 April 2023; doi:10.1038/s41589-023-01306-5 Replicative errors contribute to genetic diversity needed for evolution but in high frequency lead to genomic stability. Here, NMR is used to show via a kinetic model that DNA dynamics can determine the misincorporation of A•G and A•8OG mismatches ..read more