mRNA therapeutics have unlocked the potential for rapid responses to infectious disease, personalized treatments for cancer, and protein replacement for different diseases. To ensure efficacy, specific features need to be included in the therapeutic to promote stability, efficient translation, and to minimize endogenous regulation. In a traditional mRNA therapeutic, such as the SARS-CoV2 vaccine, the structure mirrors mRNAs found in the body. There is a cap on the 5’ end, followed by an untranslated region (UTR), the sequence encoding the therapeutic protein, a 3’ UTR, and ending with a polyA ..read more

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2023 has been an exciting year for RNA and Eclipsebio! The RNA genomics space experienced a number of successes—from fascinating publications to the Nobel Prize in Physiology or Medicine, the importance of RNA to biology and health continues to be highlighted. It has also been a year of milestones for Eclipsebio—with over 20 peer-reviewed publications citing our technologies and the successful launch of 3 new full-service solutions to continuously provide researchers with insight into RNA biology and therapeutics development. As 2023 comes to an end, we wanted to take time in this eBlog to rev ..read more

Next-generation sequencing (NGS) libraries produced by Eclipsebio’s assays typically consist of reads derived from targeted RNA regions. A visualization tool allows you to see which genes are present in your samples of interest and, depending on the assay, which specific transcript regions are enriched. To help with visualization, Eclipsebio’s service deliverables include BAM, bigWig, and BED files. Interpretation and visualization of these NGS files can be challenging, but with genomics viewers such as the Integrative Genomics Viewer (IGV), you can easily explore your data.   We covered ..read more

Next-generation sequencing (NGS) libraries produced by Eclipsebio’s assays typically consist of reads derived from targeted RNA regions. A visualization tool allows you to see which genes are present in your samples of interest and, depending on the assay, which specific transcript regions are enriched. To help with visualization, Eclipsebio’s service deliverables include BAM, bigWig, and BED files. Interpretation and visualization of these NGS files can be challenging, but with genomics viewers such as the Integrative Genomics Viewer (IGV), you can easily explore your data.   We covered ..read more

As we come towards the end of the year, we at Eclipsebio are taking time this Thanksgiving holiday to reflect on what we are grateful for. As we have asked our fellow team members what they are thankful for, two topics have come up again and again: our customers who are performing groundbreaking RNA research and the team at Eclipsebio who continuously pushes the envelope on RNA genomics solutions.  Every year our customers astonish us with how they apply our technologies to get fundamental insights into RNA biology and to develop novel life-saving therapeutics. 2023 was no exception, so ..read more

As we come towards the end of the year, we at Eclipsebio are taking time this Thanksgiving holiday to reflect on what we are grateful for. As we have asked our fellow team members what they are thankful for, two topics have come up again and again: our customers performing groundbreaking RNA research and our team who continues to push the envelope on RNA genomics solutions.  Every year our customers astonish us with how they apply our technologies to get fundamental insights into RNA biology and to develop novel life-saving therapeutics. 2023 was no exception, some of the publications f ..read more

Traditional genome analysis methods often overlook short sequences, but recent research shows that small genomic elements can have significant health and disease implications. Validating their peptide products biochemically is challenging due to their size, and conservation-based annotation is less effective for short sequences. This blog explores small functional open reading frames and their identification using ribosome profiling. Open reading frames (ORFs) are regions of the transcriptome that have an in-frame start and in-frame stop codon. This means that if a ribosome was to start transl ..read more

As a young researcher, I was always told of the terrifying tales of CLIP-seq. It was described as a wicked protocol of radiolabeled probes and grueling long days, with no guarantee of producing a viable final library. Old lab lore told of times of failure regardless of how potent the antibody or expression level of the protein were. I would shiver in my lab coat thinking about consecutive failures and the Eppendorf tube graveyard that my bench was built on.  Thankfully, I never had to run the original CLIP-seq. With the growing importance of RNA binding proteins (RBPs) and their sign ..read more

As a young researcher, I was always told of the terrifying tales of CLIP-seq. It was described as a wicked protocol of radiolabeled probes and grueling long days, with no guarantee of producing a viable final library. Old lab lore told of times of failure regardless of how potent the antibody or expression level of the protein were. I would shiver in my lab coat thinking about consecutive failures and the Eppendorf tube graveyard that my bench was built on.    Thankfully, I never had to run the original CLIP-seq. With the growing importance of RNA binding proteins (RBPs) and their si ..read more