CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) is a powerful genome editing technology that is transforming genetics and is associated with the system CRISPR-Cas9. Specifically, genome editing technology makes it possible to mutate, silence, and modify with regions of interest with high levels of precision. CRISPR coupled with Next-Generation Sequencing (NGS) enables researchers... Source ..read more

What would you do with the extra time? Next- generation sequencing (NGS) technologies have accelerated research efforts especially with multiplex PCR (mPCR). Scientists are capable of utilizing NGS to detect and analyze gene sequences in humans,animals, microorganisms, and plants for species authentication, de novo sequencing, and variant identification. Amplicon- based targeted library... Source ..read more

A liquid biopsy targets and identifies pieces of DNA from tumor cells that are circulating within the blood. This enables researchers to detect mutations in DNA, which could potentially identify various types of cancers in the early stages including lung cancer, prostate cancer, ovarian cancer, gastrointestinal cancer, colorectal cancer, and breast cancer. It is minimally invasive and therefore... Source ..read more

What is Next-Generation Sequencing (NGS)? Next-Generation Sequencing (NGS) is a massively parallel method to sequence thousands to millions of DNA molecules simultaneously. Since NGS was first introduced in 2005, it has risen to become the predominant DNA sequencing technology and heralded new levels of accessibility for research, industry, and even everyday consumers alike. Most importantly... Source ..read more

Download the PDF version of this article Introduction Next-generation sequencing (NGS) has accelerated research efforts in diverse biological fields such as molecular biology, oncology, cardiology, drug development, infectious diseases, reproductive and genetic health, agrigenomics and environmental metagenomics. Scientists routinely use NGS methods with different library preparation strategies to... Source ..read more

The tumor-suppressor protein p53 is encoded by the TP53 gene on human chromosome 17. The protein p53 has been nicknamed “The Guardian of the Genome” because of the essential role this transcription factor plays in cell division and prevention of tumor formation. Its main function lies in its response to diverse cellular stresses for regulation of gene expression for cell cycle arrest, apoptosis... Source ..read more