by Kaniz Fatema, Nur Uddin Mahmud, Dipali Rani Gupta, Md. Nurealam Siddiqui, Tahsin Islam Sakif, Aniruddha Sarker, Andrew G. Sharpe, Tofazzal Islam Endophytic bacteria, recognized as eco-friendly biofertilizers, have demonstrated the potential to enhance crop growth and yield. While the plant growth-promoting effects of endophytic bacteria have been extensively studied, the impact of weed endophytes remains less explored. In this study, we aimed to isolate endophytic bacteria from native weeds and assess their plant growth-promoting abilities in rice under varying chemical fertilization. The e ..read more

by A. Q. M. Robiul Kawser, M. Nazmul Hoque, M. Shaminur Rahman, Tahsin Islam Sakif, Tracey J. Coffey, Tofazzal Islam The field of fish microbiome research has rapidly been advancing, primarily focusing on farmed or laboratory fish species rather than natural or marine fish populations. This study sought to reveal the distinctive gut bacteriome composition and diversity within the anadromous fish species Tenualosa ilisha (hilsa), which holds the status of being the national fish of Bangladesh. We conducted an analysis on 15 gut samples obtained from 15 individual hilsa fishes collected from thr ..read more

by Alessandra De Giani, Federica Perillo, Alberto Baeri, Margherita Finazzi, Federica Facciotti, Patrizia Di Gennaro The human gut is a complex environment where the microbiota and its metabolites play a crucial role in the maintenance of a healthy state. The aim of the present work is the reconstruction of a new in vitro minimal human gut microbiota resembling the microbe-microbe networking comprising the principal phyla (Bacillota, Bacteroidota, Pseudomonadota, and Actinomycetota), to comprehend the intestinal ecosystem complexity. In the reductionist model, we mimicked the administration of ..read more

by Tomomi Sawabe, Yoshihiro Ojima, Mao Nakagawa, Toru Sawada, Yuhei O. Tahara, Makoto Miyata, Masayuki Azuma Outer membrane vesicles (OMVs) are produced by Gram-negative bacteria and deliver microbial molecules to distant target cells in a host. OMVs secreted by probiotic probiotic strain Escherichia coli Nissle 1917 (EcN) have been reported to induce an immune response. In this study, we aimed to increase the OMV production of EcN. The double gene knockout of mlaE and nlpI was conducted in EcN because the ΔmlaEΔnlpI of experimental strain E. coli K12 showed the highest OMV production in our p ..read more

by Mi Ae Park, Mirieom Park, Hyun-Jun Jang, Sung Ho Lee, Yeong Min Hwang, Soyeon Park, Donghyun Shin, Yangseon Kim Two probiotic candidates, Lactobacillus reuteri C1 (C1) and Lactobacillus acidophilus C5 (C5), which were previously isolated from canines, were evaluated in the present study. L. reuteri and L. acidophilus have anti-oxidant, anti-inflammatory, immune-enhancing, and anti-cancer properties and exhibit various probiotic effects in humans and animals. The strains C1 and C5 demonstrated good tolerance to acid and bile salt exposure, exhibited effective adhesion to HT-29 cell monolayer ..read more

by Ajay Iyer, Eva C. Soto Martín, Gary A. Cameron, Petra Louis, Sylvia H. Duncan, Charles S. Bestwick, Wendy R. Russell Many commensal gut microbes are recognized for their potential to synthesize vitamin B12, offering a promising avenue to address deficiencies through probiotic supplementation. While bioinformatics tools aid in predicting B12 biosynthetic potential, empirical validation remains crucial to confirm production, identify cobalamin vitamers, and establish biosynthetic yields. This study investigates vitamin B12 production in three human colonic bacterial species: Anaerobutyricum h ..read more

by Dayoung Kim, Jin Seok Moon, Ji Eun Kim, Ye-Ji Jang, Han Sol Choi, Ikhoon Oh This study evaluated 15 lactic acid bacteria with a focus on their ability to degrade inosine and hypo-xanthine—which are the intermediates in purine metabolism—for the management of hyperuricemia and gout. After a preliminary screening based on HPLC, Lactiplantibacillus plantarum CR1 and Lactiplantibacillus pentosus GZ1 were found to have the highest nucleoside degrading rates, and they were therefore selected for further characterization. S. thermophilus IDCC 2201, which possessed the hpt gene encoding hypoxanthin ..read more

by Julián Fernando Oviedo-León, Maribel Cornejo-Mazón, Rosario Ortiz-Hernández, Nayeli Torres-Ramírez, Humberto Hernández-Sánchez, Diana C. Castro-Rodríguez Due to the distinctive characteristics of probiotics, it is essential to pinpoint strains originating from diverse sources that prove efficacious in addressing a range of pathologies linked to dysfunction of the intestinal barrier. Nine strains of lactic acid bacteria were isolated from two different sources of tepache kefir grains (KAS2, KAS3, KAS4, KAS7, KAL4, KBS2, KBS3, KBL1 and KBL3), and were categorized to the genus Lacticaseibacill ..read more

by Razia Liaqat, Shafaq Fatima, Wajeeha Komal, Qandeel Minahal, Zakia Kanwal, Muhammad Suleman, Chris G. Carter The present study investigated the potential role of Bacillus subtilis as probiotic in striped catfish (Pangasius hypophthalmus). Fish (initial weight = 150.00±2.63g n = 180) were stocked in circular tanks. Four isonitrogenous (30%) and isolipidic (3.29%) diets were formulated having supplementation of B. subtilis at four different levels (P0; 0, P1: 1×106, P2: 1×108 and P3: 1×1010 CFU/g). Each treatment had three replicates, while each replicate had fifteen fish. The trial started o ..read more

by Yoshitaka Hashimoto, Genki Kobayashi, Noriyuki Kitagawa, Hiroshi Okada, Masahide Hamaguchi, Michiaki Fukui Background This randomized, parallel-group study aims to investigate the effects of the probiotic Bifidobacterium bifidum G9-1 (BBG9-1) on the symptoms of diarrhea or constipation in patients with type 2 diabetes mellitus (T2DM). Methods This study will examine 100 patients with T2DM who suffering from symptoms of diarrhea or constipation. Eligible patients will be randomly assigned 1:1 to two groups (group A, BBG9-1 group; group B, control group), after the baseline examination. Patie ..read more