
MicrobeHunter Microscopy Forum
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What is your microscopy history? What equipment do you use? Talk about it all here by posting pictures and descriptions of your microscopes. Also, discuss different microscopic techniques and illumination methods, such as Brightfield, Darkfield, Phase Contrast, DIC, Oblique illumination, and more.
MicrobeHunter Microscopy Forum
12h ago
When you say you can't get the sensor in the right spot, you mean to be parfocal with the eyepieces correct? Because as you mentioned, it positions the sensor too "far away" to be parfocal.
Yes, exactly. And of course if you just refocus to move the image circle knto the sensor you theoretically are losing some optical quality since you're pushing it out of spec, though I have not tested whether it actually makes a noticeable difference in this case.
Statistics: Posted by Scarodactyl — Sun Dec 10, 2023 11:14 pm ..read more
MicrobeHunter Microscopy Forum
12h ago
Here are some shots. I have used an adapter to mount my T3I directly to the camera port. Due to the distance to the cameras sensor, as mentioned, it is not parfocal with the eyepieces. However, given I generally tether my camera to my laptop and do not use the eyepieces when taking pics, the fact it is not parfocal does not bother me.
Statistics: Posted by einman — Sun Dec 10, 2023 11:10 pm ..read more
MicrobeHunter Microscopy Forum
14h ago
Sorry I haven't read everything in detail. But have you thought about the position of the interference fringe relative to the back focal plane of your objectives? I've measured the location of the BFPs of some of the objectives for which your prisms were made. The data is here:
https://www.photomacrography.net/forum/ ... 45#p285745
I haven't played with slit condenser DIC, so I'm not entirely sure, but I'm thinking that the gradient your seeing could be caused by a mismatch in the interference fringe position. Your slit will be protected at the back focal plane, and I assume that's where the ..read more
MicrobeHunter Microscopy Forum
14h ago
Hi Sure Squinsalot.: Until now I have only test with the cf40X 160mm 0,65NA nikon objective, and BD 40 epi prism, with the 20X PZO condenser prism.
I hope next weekend I can try with the cf 20X
What are you using in the nosepiece place?, what kind of prisma?
I use the BD epi DIC optipht Nikon nosepiece. If yours isn´the same, my results couldn´t match with yours...
Best regards
Statistics: Posted by Jose Antonio Mena — Sun Dec 10, 2023 9:04 pm ..read more
MicrobeHunter Microscopy Forum
14h ago
It comes down to two things: how big the image circle is and where it's positioned. By default the smz-u puts out an image that's a bit over 27mm in diameter, which is actually larger than it was specced for. If you put an aps-c sensor with a ~27mm diagonal right on the image you get a nice image that matches what you see in the eyepieces really well. The problem is that the image is positioned like 20ish mm above the edge of thr cemra port. A dslr has like 40mm of space between the lens mount and the sensor to accomodate the mirror so it just physically can't get the sensor in the right spot ..read more
MicrobeHunter Microscopy Forum
14h ago
The microscope is no longer for sale (went on %Bay). Cheers.
Statistics: Posted by erikwetterskog — Sun Dec 10, 2023 8:57 pm ..read more
MicrobeHunter Microscopy Forum
14h ago
Hola Jose....do you have a sample DIC microscopy image with that condenser side 20x DIC prism AND 20x objective with its DIC prism engaged? I'm considering a condenser modification like yours for my Optiphot, but I'd like to see if its' better than my Sanderson plate. I suspect it is, but it would be nice to see, and be certain.
Statistics: Posted by Sure Squintsalot — Sun Dec 10, 2023 8:51 pm ..read more
MicrobeHunter Microscopy Forum
14h ago
Could you elabroate a bit on that? I'm very happy you got it to work!
Statistics: Posted by Scarodactyl — Sun Dec 10, 2023 8:31 pm ..read more
MicrobeHunter Microscopy Forum
14h ago
Thank you very much to everyone who gave me advice, I have achieved it, now it looks perfectly and the zoom works well. My problem was with the lenses, which when they sold it to me, at a very good price, were installed incorrectly. The head was in perfect condition.
Regards.
Statistics: Posted by stjepo — Sun Dec 10, 2023 8:25 pm ..read more
MicrobeHunter Microscopy Forum
16h ago
It comes down to two things: how big the image circle is and where it's positioned. By default the smz-u puts out an image that's a bit over 27mm in diameter, which is actually larger than it was specced for. If you put an aps-c sensor with a ~27mm diagonal right on the image you get a nice image that matches what you see in the eyepieces really well. The problem is that the image is positioned like 20ish mm above the edge of thr cemra port. A dslr has like 40mm of space between the lens mount and the sensor to accomodate the mirror so it just physically can't get the sensor in the right spot ..read more