FocalPlane
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FocalPlane is your microscopy community site, hosted by Journal of Cell Science (JCS) and encompassing all fields in the biological sciences where they meet microscopy. The website includes primers on new techniques and interviews with the people developing them, technique validation and short video tutorials. We are also featuring case studies in which experts in microscopy and image analysis..
FocalPlane
1d ago
Our featured image, acquired by Julien Rességuier, shows the branchial cavity lymphoid architecture of an adult zebrafish. It is a 2D projection of a 3D multi-field of view acquisition of a transversal cryosection (30 μm) that has been stained with fluorescent phalloidin (inverted LUT – grey/copper) and DAPI (blue) to reveal tissue structures and labelled with an anti-ZAP70 antibody to reveal T / natural killer (NK) cells (cyan ..read more
FocalPlane
1w ago
In this post, we share preprints that use microscopy tools to answer questions in biology ..read more
FocalPlane
1w ago
A widefield microscope can be utilised for far more than simple fluorescence experiments. By carefully choosing peripheral components including the light source, the system can open up an array of additional applications, from FRET to optogenetic stimulation and calcium imaging. LEDs are the go-to method for microscope illumination, offering many benefits in terms of performance ..read more
FocalPlane
1w ago
We are delighted to announce that we are hosting an online workshop on science communication. The workshop will be split into sections on oral communication and written communication and includes a three-minute research talk competition and collaborative writing sprints ..read more
FocalPlane
3w ago
We are delighted to launch the FocalPlane-elmi2024 image competition. Enter your favourite microscopy images for your chance to win £150 and see your image on the cover of Journal of Cell Science. Voting for the shortlisted images will begin the week before elmi2024 and continue through the conference, until we announce the winner on Friday ..read more
FocalPlane
3w ago
Imagine you try out a software tool for the analysis of your data and for some reason it is not working in the way you expected. Neither the user documentation, nor forums like image.sc or stack overflow can provide answers. Then it is likely that you either found a bug in the software tool or ..read more
FocalPlane
3w ago
Over the past two decades, high profile research institutions have come to rely on distinct core facilities for much of their data and image analysis. The benefits of the newest technologies are often softened by the costs of purchasing and maintaining such equipment for regular use. For microscopy and imaging, it only made sense that ..read more
FocalPlane
1M ago
As I explained in my previous post, this journey began because I needed to learn how to master QuPath. I turned it into a vlogging affair to be held accountable, help others, and use it to find common user problems. For the last six weeks I have explored all the ways QuPath can easily help ..read more
FocalPlane
1M ago
In our upcoming FocalPlane features... webinar, we're focussing on microscopy education with talks from Jennifer Waters (Director of the Nikon Imaging Center and Lecturer in Cell Biology at Harvard Medical School) and Robert Haase (Training Coordinator at ScaDS.AI, Leipzig University ..read more
FocalPlane
1M ago
Community surveys often point to the biggest bottleneck in excellent bioimaging science being image analysis. That’s why the RMS DAIM committee and their friends across the world are putting on another event to highlight the fantastic image.sc forum. All the info you need is here: Image.sc LIVE around the world! – Announcements – Image.sc Forum and in the poster ..read more