Green Fluorescent Blog
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My name is Gal Haimovich. When I started this blog I was a postdoc at Rob Singer's lab, a leading laboratory that uses advanced fluorescent microscopy techniques to study regulation of gene expression. I will discuss here basic topics like fluorescent molecules, fluorescent proteins, old and new fluorescent microscopy techniques and their applications in biology. In between, I'll..
Green Fluorescent Blog
6M ago
There are at least 170 known chemical modifications on RNA molecules in all organisms. Most of these modifications are on tRNAs, rRNAs and small RNAs. But over the past two decades, researchers found that eukaryotic mRNAs also carry some modifications ..read more
Green Fluorescent Blog
1y ago
Processing bodies (P-bodies, PBs) are aggregates of mRNA degradation proteins, suggested 20 years ago to be sites of mRNA degradation. Later work provided evidence to the contrary, and the function of PBs remained controversial. A new pre-print from Bin Wu’s ..read more
Green Fluorescent Blog
1y ago
An assumption in microscopy and other methods is that chemical fixation of the cells is fast enough not to affect the biological process we study. A new paper in eLife shows that fixation affects the appearance of phase-separated proteins. In ..read more
Green Fluorescent Blog
1y ago
Due to Twitter becoming a garbage can website, with prospect of collapsing, I’m copying here my previous conferences live-tweets. I’ll do it gradually from last to first and I might edit it to look pretty later-on. Israeli RNA society meeting ..read more
Green Fluorescent Blog
1y ago
A group of researchers from Norway found that mCherry – and possibly other fluorescen proteins – has a shorter translated isoform in bacteria. This could affect localization and expression studies. mCherry is a widely used fluorescent protein. It is derived ..read more
Green Fluorescent Blog
1y ago
A new preprint from Brian Zid’s lab shows accumulation of P-body-like aggregates in old yeast cells with a suggested pathological role in aging. I think some key experiments are missing. A short review. We do not yet understand the aging ..read more
Green Fluorescent Blog
1y ago
Three recent papers begin to explore the dynamics of miRNA translation repression and mRNA decay at the single-molecule resolution. One paper contradicts the others. Our understanding of the regulation on mRNA molecules has advanced a lot thanks to single-molecule imaging ..read more
Green Fluorescent Blog
1y ago
A year and a half ago I wrote about a preprint showing that small RNA molecules are being glycosylated. Now this paper is published in Cell, with some additional data. In particular, the work from Carolyn Bertozzi’s lab and led ..read more
Green Fluorescent Blog
1y ago
I write this entry to accompany my short talk at Woodstock.bio meeting ( #physiologicalirrelevantconference ) . A few years ago we published a paper in PNAS in which we showed that full-length mRNAs transfer between mammalian cells via a unique ..read more
Green Fluorescent Blog
1y ago
A new pre-print from Caroline Bertozzi’s lab shows that some RNA molecules are glycosylated. At least some of these glyco-RNA molecules might reside inside the ER lumen. There are four major types of bio-molecules: proteins, nucleic acids, lipids and sugars ..read more