CRISPR-Cas9 represents one of the most impactful discoveries in the field of genetic engineering and medicine of recent times. Its discovery catalyzed a paradigm shift in the treatment approach to many genetic diseases, paving the way to simple and effective gene therapy for a large class of diseases.   Despite its... The post Towards safe and precise gene therapies: revealing the impact of DNA Supercoiling on CRISPR-Cas9 specificity appeared first on LUMICKS ..read more

Surprising insights into the roles of membranes and the actin cytoskeleton in cellular tension propagation – congratulations to all authors for successfully implementing a new and elegant assay with the C-Trap!   There was a long-standing dispute in the scientific community about whether the actin cortex facilitates or impedes tension propagation... The post Cell mechanics with the C-Trap – cellular tension propagation revisited appeared first on LUMICKS ..read more

​​​PICH (Plk1-interacting checkpoint helicase) belongs to the SNF2 family of ATP-dependent chromatin remodelers. However, what makes PICH unique is that it lacks a nucleotide-binding domain despite having a great affinity for bare DNA. Because of this molecular structure, it has been hypothesized that the binding of PICH to chromatinized bridges... The post The C-Trap uncovers new insights into the mechanisms for ATP-dependent chromatin remodeling appeared first on LUMICKS ..read more

​​​PICH (Plk1-interacting checkpoint helicase) belongs to the SNF2 family of ATP-dependent chromatin remodelers. However, what makes PICH unique is that it lacks a nucleotide-binding domain despite having a great affinity for bare DNA. Because of this molecular structure, it has been hypothesized that the binding of PICH to chromatinized bridges... The post The C-Trap uncovers new insights into the mechanisms for ATP-dependent chromatin remodeling appeared first on LUMICKS ..read more

We are excited to announce that LUMICKS is donating laboratory equipment for a summer course in Yobe, Nigeria, organized by the Biomedical Science Research and Training Centre (BioRTC) and Trend In Africa. We added various tools from our LUMICKS C-Trap® head quarters to the donation. Nigeria has a huge shortage... The post Putting our unused lab equipment to better use appeared first on LUMICKS ..read more

New insights into the regulation of transcription termination have been revealed by researchers from the lab of Shixin Liu at The Rockefeller University, New York, in a recent publication in Molecular Cell. Using the powerful LUMICKS C-Trap® technology that allows the direct observation of molecular mechanisms, they were able to... The post C-Trap^® experiments reveal novel mechanism for transcription termination in bacteria  appeared first on LUMICKS ..read more

A recent study published in Nature Communications led by Shixin Liu from The Rockefeller University, New York, demonstrates how the C-Trap® can be used to directly visualize and observe molecular mechanisms in action, leading to better and faster insights into critical molecular processes. This study marks an important milestone for LUMICKS,... The post 100th study using the C-Trap^® provides crucial insights into how the Smc5/6 complex stabilizes certain DNA structures   appeared first on LUMICKS ..read more

In two recent scientific publications, Johannes Stigler and his team from the Gene Center of the Ludwig-Maximilians-University Munich, Germany, set out to mechanistically describe the action of the Structural Maintenance of Chromosome (SMC) Complexes using LUMICKS’ C-Trap® technology. By performing dynamic single-molecule experiments coupled with real-time visualization, they were able... The post C-Trap® experiments shed light on the mechanisms that govern Structural Maintenance of Chromosome (SMC) Complexes   appeared first on LUMICKS ..read more

For a long time, scientists have been striving to understand how DNA-binding proteins interact with DNA substrates in real time at the single-molecule level, using a variety of imaging techniques and optical platforms. However, for most of these methods, scientists always had to choose between studying purified proteins under specific... The post Meet SMADNE, a revolutionary new technique to unlock the secrets of DNA-binding proteins  appeared first on LUMICKS ..read more

Double-stranded breaks (DSBs) are among the most serious and lethal types of DNA damage known that can lead to cell death and cancer. POLQ is an enzyme in humans that we know plays a crucial role in DSB repair and is overexpressed in many types of cancer, promoting cancer cell... The post C-Trap^® experiments highlight the potential of POLQ inhibitors as BCRA-deficient cancer therapeutics appeared first on LUMICKS ..read more