Spectral Comparison: Raman Spectroscopy vs. FTIR Spectroscopy  Raman Spectroscopy and Fourier-Transform Infrared (FTIR) Spectroscopy stand out as two prominent techniques offering invaluable insights into molecular structures. While Raman Spectroscopy utilizes the inelastic scattering of monochromatic light to provide detailed information about vibrational modes, FTIR Spectroscopy relies on the absorption of infrared radiation, unveiling molecular composition. Both methods play crucial roles in various industries and applications. This comparative analysis sheds light on their differenc ..read more

How to Leverage Python to Maximize Raman Spectroscopy We have two technical notes on Raman and Python tailored for scientists and engineers well-versed in both Python and Raman spectroscopy within academic settings or diverse industrial domains. These resources are crafted to meet the specific needs of professionals possessing a foundational understanding of these realms. Whether you operate in the academic sphere or diverse industrial sectors, the content is curated to resonate with individuals who have a background in Python programming and practical experience in the intricacies of Raman ..read more

UPSTREAM BIOPROCESSING APPLICATIONS WITH TORNADO’S RAMAN SOLUTIONS. Mammalian Cell Culture Monitoring And Control Originating with the development of stable Chinese Hamster Ovary (CHO) cell lines, the technology of mammalian cell culture has garnered considerable interest and support from Process Analytical Technology (PAT) scientists globally, and for good reasons. To ensure a robust process and production yield of the desired protein, careful monitoring of crucial analytes and process parameters is essential. In the past, these analytes and parameters required frequent sampling and offline ..read more

The United States, Food and Drug Administration (FDA) Conducts a Comprehensive study using Tornado’s HTVS-enabled Hypeflux PRO Plus (HFPP) and Bioimmersion probe.   The study, Raman Spectrometry as a tool to study minimization of batch age effects and make product quality decisions for biotherapeutic antibody production, was shared at the 2023 FDA Science Forum at the Product Development and Manufacturing session.     The study illustrates the value of Raman Spectroscopy as a relevant cost-saving PAT tool while elucidating methods to enhance or achieve superior outcomes ..read more

The Value of Raman Spectroscopy for Process Monitoring and Control The last post laid out reasons why Raman is a good tool for Process Understanding. In many cases, if a tool adds value for process understanding in development, it can also add value for process monitoring and process control. This is the basis and context for this post. What are the key points to consider when deciding whether to deploy Raman as a process monitoring tool, with the possible goal of using it to its maximum potential as a process control technology with full loop feedback control? In this post, we will discuss ..read more

The Value of Raman Spectroscopy An illustration that comes to mind, on the importance of process understanding, is one I heard at a job interview over 30 years ago. The knowledge in the world as it relates to you as an individual can be divided into four segments: Things that you know that you know. things that you know that you don’t know. things that don’t know that you know. things that you don’t know that you don’t know.   This same categorization of knowledge to your process can be applied, as seen in the chart below. The proportions of each category may not be equal, but the p ..read more

Webinar – What’s New at Tornado: Advances in Process Raman Deployment Tornado has some exciting developments in the pipeline for 2022 to strengthen and build our market in Raman spectroscopy. Please join Mark Kemper, Director of Applications Development and Customer Success, in Tornado’s first webinar of the year What’s New at Tornado: Advances in Process Raman Deployment and have your questions answered on March 1 at 9am and 9pm EST. Tornado Spectral Systems is an innovator in process Raman spectroscopy. As developers of the game-changing High Throughput Virtual Slit (HTVS) technology ..read more

Identifying Polymorphism with In-Situ Raman Spectroscopy at Screening Scale Please join Mark Kemper and our valued partner Dr. Thomas Kendall from Technobis Crystallization Systems as we co-host the webinar Identifying Polymorphism with In-Situ Raman Spectroscopy at Screening Scale and answer your questions next month on March 8. Polymorphism in pharmaceutical active compounds (API) play a crucial role in final particle properties such as bioavailability, morphology, and dissolution rate with direct effect on important downstream decisions. Several methods can be used to detect and different ..read more

Tracking Polymorph & Crystal Morphology Crystallization is a commonly used process for solid-dose manufacturing. To improve the biological performance and economic benefits of a crystallization process, proper process control is necessary. However, crystallization can be difficult to control due to the complex relationship between thermodynamic and kinetic factors and processes such as nucleation, growth, and agglomeration. A thorough understanding of the process is required, which can be done through modeling, design of experiment stress testing and the use of process analytical technol ..read more

Probe-Based Raman Spectroscopy When chemists and spectroscopists want to measure molecules and molecular bonds in a chemical or biological sample, they may use Raman Spectroscopy, which uses laser light to observe changes in the vibrational energies of their chemical systems/samples. Since the development of the first commercial Raman analyzer in 1953, there have been advances with lasers and detectors and the discovery of new phenomena have expanded the use of Raman in several fields such as pharmaceuticals and chemical manufacturing. But what about the Raman probe? There have been many adv ..read more