BioTech, Vol. 13, Pages 10: Transcriptional and Metabolic Response of a Strain of Escherichia coli PTS− to a Perturbation of the Energetic Level by Modification of [ATP]/[ADP] Ratio BioTech doi: 10.3390/biotech13020010 Authors: Sandra Soria Ofelia E. Carreón-Rodríguez Ramón de Anda Noemí Flores Adelfo Escalante Francisco Bolívar The intracellular [ATP]/[ADP] ratio is crucial for Escherichia coli’s cellular functions, impacting transport, phosphorylation, signaling, and stress responses. Overexpression of F1-ATPase genes in E. coli increases glucose consumption, lowers e ..read more

BioTech, Vol. 13, Pages 9: Synthesis and Biological Activities of Some Metal Complexes of Peptides: A Review BioTech doi: 10.3390/biotech13020009 Authors: Petja Marinova Kristina Tamahkyarova Peptides, both natural and synthetic, are well suited for a wide range of purposes and offer versatile applications in different fields such as biocatalysts, injectable hydrogels, tumor treatment, and drug delivery. The research of the better part of the cited papers was conducted using various database platforms such as MetalPDB. The rising prominence of therapeutic peptides encompasses anticancer, antiv ..read more

BioTech, Vol. 13, Pages 8: Novel Pseudomonas Species Prevent the Growth of the Phytopathogenic Fungus Aspergillus flavus BioTech doi: 10.3390/biotech13020008 Authors: Franciene Rabiço Tiago Cabral Borelli Robson Carlos Alnoch Maria de Lourdes Teixeira de Moraes Polizeli Ricardo R. da Silva Rafael Silva-Rocha María-Eugenia Guazzaroni In response to the escalating demand for sustainable agricultural methodologies, the utilization of microbial volatile organic compounds (VOCs) as antagonists against phytopathogens has emerged as a viable eco-friendly alternative. Microbial volatiles exhibit rapid ..read more

BioTech, Vol. 13, Pages 7: Optimization of Aqueous Extraction of Polyphenols from Cuminum cyminum Seeds Using Response Surface Methodology and Assessment of Biological Activity BioTech doi: 10.3390/biotech13010007 Authors: Hana El Tannir Diana Houhou Espérance Debs Mohamed Koubaa Adla Jammoul Bilal Azakir Mahmoud I. Khalil Nada El Darra Nicolas Louka (1) Background: Cumin seeds, extracted from the plant Cuminum cyminum, are abundant in phenolic compounds and have been extensively researched for their chemical makeup and biological effects. The objective of this research is to enhance the water ..read more

BioTech, Vol. 13, Pages 6: Effect of Chitosan Degradation Products, Glucosamine and Chitosan Oligosaccharide, on Osteoclastic Differentiation BioTech doi: 10.3390/biotech13010006 Authors: Tomoharu Takeuchi Midori Oyama Tomomi Hatanaka Chitosan, a natural cationic polysaccharide derived from crustaceans and shellfish shells, is known for its advantageous biological properties, including biodegradability, biocompatibility, and antibacterial activity. Chitosan and its composite materials are studied for their potential for bone tissue repair. However, the effects of chitosan degradation products ..read more

BioTech, Vol. 13, Pages 5: Susceptibility of Tetranychus urticae to the Alkaloidal Extract of Zanthoxylum schreberi Bark: Phenotypic and Biochemical Insights for Biotechnological Exploitation BioTech doi: 10.3390/biotech13010005 Authors: Ricardo A. Rincón Daniel Rodríguez Ericsson Coy-Barrera Tetranychus urticae Koch, a phytophagous mite, is one of the most significant crop pests globally. The primary method employed for controlling T. urticae involves chemical means, utilizing synthesized products, posing the risk of developing resistance. The urgency for novel strategies integrated into pest ..read more

BioTech, Vol. 13, Pages 4: Expression and Purification of Cp3GT: Structural Analysis and Modeling of a Key Plant Flavonol-3-O Glucosyltransferase from Citrus paradisi BioTech doi: 10.3390/biotech13010004 Authors: Aaron S. Birchfield Cecilia A. McIntosh Glycosyltransferases (GTs) are pivotal enzymes in the biosynthesis of various biological molecules. This study focuses on the scale-up, expression, and purification of a plant flavonol-specific 3-O glucosyltransferase (Cp3GT), a key enzyme from Citrus paradisi, for structural analysis and modeling. The challenges associated with recombinant prot ..read more

BioTech, Vol. 13, Pages 3: Exploring DNA Damage and Repair Mechanisms: A Review with Computational Insights BioTech doi: 10.3390/biotech13010003 Authors: Jiawei Chen Ravi Potlapalli Heng Quan Lingtao Chen Ying Xie Seyedamin Pouriyeh Nazmus Sakib Lichao Liu Yixin Xie DNA damage is a critical factor contributing to genetic alterations, directly affecting human health, including developing diseases such as cancer and age-related disorders. DNA repair mechanisms play a pivotal role in safeguarding genetic integrity and preventing the onset of these ailments. Over the past decade, substantial progr ..read more

BioTech, Vol. 13, Pages 2: Styrene Production in Genetically Engineered Escherichia coli in a Two-Phase Culture BioTech doi: 10.3390/biotech13010002 Authors: Shuhei Noda Ryosuke Fujiwara Yutaro Mori Mayumi Dainin Tomokazu Shirai Akihiko Kondo Styrene is an important industrial chemical. Although several studies have reported microbial styrene production, the amount of styrene produced in batch cultures can be increased. In this study, styrene was produced using genetically engineered Escherichia coli. First, we evaluated five types of phenylalanine ammonia lyases (PALs) from Arabidopsis thalia ..read more

BioTech, Vol. 13, Pages 1: Gene Therapy for Genetic Syndromes: Understanding the Current State to Guide Future Care BioTech doi: 10.3390/biotech13010001 Authors: Marian L. Henderson Jacob K. Zieba Xiaopeng Li Daniel B. Campbell Michael R. Williams Daniel L. Vogt Caleb P. Bupp Yvonne M. Edgerly Surender Rajasekaran Nicholas L. Hartog Jeremy W. Prokop Jena M. Krueger Gene therapy holds promise as a life-changing option for individuals with genetic variants that give rise to disease. FDA-approved gene therapies for Spinal Muscular Atrophy (SMA), cerebral adrenoleukodystrophy, β-Thala ..read more