A comparison of different intensified upstream processes highlighting the advantage of WuXi Biologics' Ultra‐high Productivity platform (WuXiUPTM) in improved product quality and purification yield
Wiley Online Library » Biotechnology Progress
by Xiang Zheng, Mingyue Fang, Yanling Zou, Shuo Wang, Weichang Zhou, Hang Zhou
4d ago
Abstract WuXiUPTM, WuXi Biologics’ Ultra-high Productivity platform, is an intensified and integrated continuous bioprocess platform developed for production of various biologics including monoclonal antibodies, fusion proteins, and bispecific antibodies. This process technology platform has manifested its remarkable capability in boosting the volumetric productivity of various biologics and has been implemented for large-scale clinical material productions. In this paper, case studies of the production of different pharmaceutical proteins using two high-producing and intensified culture modes ..read more
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Rapid total sialic acid monitoring during cell culture process using a machine learning model based soft sensor
Wiley Online Library » Biotechnology Progress
by Amir M. Behdani, Yuxiang Zhao, Grace Yao, Dhanuka Wasalathanthri, Eric Hodgman, Michael Borys, Gloria Li, Anurag Khetan, Dayanjan Wijesinghe, Anthony Leone
1w ago
Abstract Total sialic acid content (TSA) in biotherapeutic proteins is often a critical quality attribute as it impacts the drug efficacy. Traditional wet chemical assays to quantify TSA in biotherapeutic proteins during cell culture typically takes several hours or longer due to the complexity of the assay which involves isolation of sialic acid from the protein of interest, followed by sample preparation and chromatographic based separation for analysis. Here, we developed a machine learning model-based technology to rapidly predict TSA during cell culture by using typically measured process ..read more
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Biotechnological potential of yeast cell wall: An overview
Wiley Online Library » Biotechnology Progress
by Fanny Machado Jofre, Sarah de Souza Queiroz, Diana Alva Sanchez, Priscila Vaz Arruda, Júlio César dos Santos, Maria das Graças de Almeida Felipe
1w ago
Abstract The yeast cell wall is a complex structure whose main function is to protect the cell from physical and chemical damage, providing it with rigidity. It is composed of a matrix of covalently linked polysaccharides and proteins, including β-glucans, mannoproteins, and chitin, whose proportion can vary according to the yeast species and environmental conditions. The main components of the yeast cell wall have relevant properties that expand the possibilities of use in different industrial sectors, such as pharmaceutical, food, medical, veterinary, and cosmetic. Some applications include ..read more
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Cover
Wiley Online Library » Biotechnology Progress
by Hong Li, Patricia Rose, Patricia Rowicki, Collette Cutler, Jeffrey T. McPhee, Claudia Frey, Linda Lemieux, Gerald Pelette, Joo Kok Ang, Ren Liu, Douglas D. Richardson
2w ago
The cover image is based on the Research Article Advancing multiproduct resin reuse for development and clinical manufacturing of an antibody-based therapeutic by Hong Li et al., https://doi.org/10.1002/btpr.3434 Cover illustration created by Laura Maaske ..read more
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Issue Information
Wiley Online Library » Biotechnology Progress
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2w ago
Biotechnology Progress, Volume 40, Issue 3, May/June 2024 ..read more
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Modeling of cell cultivation for monoclonal antibody production processes considering lactate metabolic shifts
Wiley Online Library » Biotechnology Progress
by Kozue Okamura, Sara Badr, Yusuke Ichida, Akira Yamada, Hirokazu Sugiyama
2w ago
Abstract Demand for monoclonal antibodies (mAbs) is rapidly increasing. To achieve higher productivity, there have been improvements to cell lines, operating modes, media, and cultivation conditions. Representative mathematical models are needed to narrow down the growing number of process alternatives. Previous studies have proposed mechanistic models to depict cell metabolic shifts (e.g., lactate production to consumption). However, the impacts of variations of some operating conditions have not yet been fully incorporated in such models. This paper offers a new mechanistic model considering ..read more
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Primary clarification of mammalian cell culture fluid using enhanced sedimentation on inclined surfaces inside the single‐use disposable Sudhin BioSettler150
Wiley Online Library » Biotechnology Progress
by Christopher B. Glascock, Dhinakar S. Kompala
3w ago
Abstract The first downstream processing step in the purification of a biopharmaceutical protein secreted into mammalian cell culture fluid is the primary clarification of the culture fluid. As cell densities in the fed-batch and increasingly more common perfusion bioreactors have increased over last two decades through intensified upstream bioreactor production processes, the traditional primary clarification unit operations of centrifugation and/or microfiltration become more challenging with issues like frequent desludging, cell disruption due to shear damage and quick fouling of membranes ..read more
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The development of an automated microscope image tracking and analysis system
Wiley Online Library » Biotechnology Progress
by Lillian McAfee, Zach Heath, William Anderson, Marvin Hozi, John Walker Orr, Youngbok (Abraham) Kang
3w ago
Abstract Microscopy image analysis plays a crucial role in understanding cellular behavior and uncovering important insights in various biological and medical research domains. Tracking cells within the time-lapse microscopy images is a fundamental technique that enables the study of cell dynamics, interactions, and migration. While manual cell tracking is possible, it is time-consuming and prone to subjective biases that impact results. In order to solve this issue, we sought to create an automated software solution, named cell analyzer, which is able to track cells within microscopy images w ..read more
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Evaluation of TrpM and PsiD substrate promiscuity reveals new biocatalytic capabilities
Wiley Online Library » Biotechnology Progress
by Fiona C. Kanis, Caroline N. Broude, Elle B. Hellwarth, William J. Gibbons Jr, Abhishek K. Sen, Alexandra M. Adams, Xin Wang, J. Andrew Jones
3w ago
Abstract N-methylated tryptamines, such as the hallucinogenic natural products, psilocybin and N,N-dimethyltryptamine (DMT), are gaining interest from the medical community due to their potential as next generation treatments for mental health disorders. The clinical relevance of these compounds has driven scientists to develop biosynthetic production routes to a number of tryptamine drug candidates, and efforts are ongoing to expand and further develop these biosynthetic capabilities. To that end, we have further characterized the substrate preferences of two enzymes involved in tryptamine bi ..read more
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Metagenomics analysis yields assembled genomes from prokaryotic anaerobes with polymer‐degrading potential
Wiley Online Library » Biotechnology Progress
by Elaina M. Blair, Jennifer L. Brown, Dong Li, Patricia A. Holden, Michelle A. O'Malley
3w ago
Abstract Anaerobic microbial communities are often highly degradative, such as those found in the herbivore rumen and large-scale anaerobic digesters. Since the microbial communities in these systems degrade recalcitrant organic polymers, we hypothesize that some microbes in anaerobic environments may be involved in man-made plastic association, deformation, or even breakdown. While efforts have been put toward characterizing microbial communities, many microbes remain unidentified until they can be sufficiently cultivated to generate enough genetic material to assemble high-quality metagenome ..read more
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