Staphylococcus aureus sequencing
Science Forums.net » Microbiology and Immunology Latest Topics
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1w ago
I'm a beginner when it comes to S.aureus. Can anyone explain to me some terms related to S. aureus sequencing such as Cluster complex, spa type, SCCmec, Clonal complex, accressory gene Regular? And some information about popular types of spas in humanistic medicine and veterinary medicine. Thank for all ..read more
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Is the copy number of combined vectors equal in E. coli cells?
Science Forums.net » Microbiology and Immunology Latest Topics
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8M ago
I have a series of combination plasmids based on the pET-28a vector, each of which contains the ppk gene from different bacteria inserted at the same site. The sequence length of these plasmids is approximately 7300 base pairs each. I am curious about whether the copy numbers of these plasmids remain consistent after they are separately introduced into E. coli cells ..read more
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Germs on laptop keyboard
Science Forums.net » Microbiology and Immunology Latest Topics
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9M ago
I'm probably worrying about nothing but since the pandemic I've been even more worried about germs. I was given a used laptop - the keyboard I sprayed lightly with an alcohol aerosal and then wiped down with antibacterial wipes. I didn't take the keys off, so I'm not confident that the underneath of the keys got cleaned. How long do harmful germs last on a keyboard anyway? I've had it for about 2 months and since I don't really understand microbiology and have only seen the scaremongering in adverts about germs, I'm freaking out. I didn't wait for the alcohol spray to dry either before I wiped ..read more
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Can I conclude the mode of action of an antimicrobial compounds in the results?
Science Forums.net » Microbiology and Immunology Latest Topics
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10M ago
I am writing my thesis/dissertation. It dealt with the antimicrobial activity of novel compounds. Next to determining the MIC, I also subcultured the organisms to conclude about the mode of action. I determined the MBCs for every compound and also calculated the MBC/MIC ratio. This ratio tells me more about the potential mode of action (static or cidal). For instance, smaller than 4 would be bactericidal. I don't know how to deal with these results. Can I already assign the mode of action after naming the results, or should this be part of the discussion? I had the same question about my syn ..read more
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Malaria and Nicotine
Science Forums.net » Microbiology and Immunology Latest Topics
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1y ago
I've been a heavy smoker since I was 19. Just background fact. After maybe 17 malaria-free years in Nigeria, I eventually came down with it in 2016 (P. falciparum) and running 400 C I was immediately hospitalised for 3 days on a combination quinine/paracetamol drip. This repeated 2 years later. On both occasions, I had three days of no urge to smoke whatsoever, which was a really big deal to me. Normally two hours abstention pushes me into a tense, hyperactive somewhat sociopathic state that can alarm those around me. Basically. I smoke to stay in employment (and married!). So any way out ..read more
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Does microwave kill bacteria?
Science Forums.net » Microbiology and Immunology Latest Topics
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1y ago
I think this is a fairly simple question, but I'm interested in such research. If you heat yogurt in the microwave, will the bacteria in it die or not? I understand that this is a very simple question, so don't laugh, but I didn't find the answer. Some people think that the bacteria will die and the milk will lose its beneficial properties, others think that everything will be fine, what do you think? I also wanted to add at the same time why the immune system does not kill beneficial bacteria in our intestines? If the second question is difficult then just give a link to a book on the subject ..read more
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Anyone know what these spots are?
Science Forums.net » Microbiology and Immunology Latest Topics
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1y ago
Can someone help identify these spots?   ..read more
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Viruses replace antibiotics
Science Forums.net » Microbiology and Immunology Latest Topics
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1y ago
Gradually, the bacteria adapt to antibiotics. In the nature of bacteria, viruses sometimes win. In the future, when antibiotics stop working on bacteria, we should be ready to use viruses. Do you think this is possible ..read more
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Human blood at 200 - 400x. What is the unidentified object?
Science Forums.net » Microbiology and Immunology Latest Topics
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1y ago
This is a photograph of a human blood stain on an organic (inert) substrate.  As I am no expert in this field, what am I looking at? and what is the "winding" in the middle of the photo.. does blood present like this under magnification?   ..read more
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Genetics
Science Forums.net » Microbiology and Immunology Latest Topics
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1y ago
I have recently had genetic testing to rule out BRCA gene, but came up with a PTEN mutation (p.Y336... c.1008C>G)- tumor suppressant gene . I have a medical history of a tiny breast cancer about 12 years ago (a few millimeters), colon polyps, and macrocephaly. Other than that I am perfectly healthy.  My physicians have told  me I have Cowden syndrome, and it is now a 4 alarm fire with need for multiple tests per year, but all the studies I read on this indicate incidence is about 1/200,000. Not sure about the actual pathogenicity of this, as they are just reporting case studies an ..read more
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