ENABLING HIV EPIDEMIOLOGY Simplifying HIV Variant Tracking with Low Throughput Automation “Lab A” is an epidemiology lab tracking variants of the human immunodeficiency virus (HIV). They collect samples from approximately 16 people a day, amplify the whole viral genome with specific primers and the amplicons obtained are sequenced. An amplicon-based approach for whole genome sequencing (WGS) facilitates the analysis of complete viral genomes allowing the identification of new and existing variants. This analysis is used for tracking, establishing transmission routes, and outbreak control ..read more

SINGLE CELL SEQUENCING SERVICES LEVERAGING THE HIVE scRNAseq SOLUTION December 7th, 2022  |  Sean Banks, Director, Business Development at Psomagen Can you tell us about single cell RNA-seq and other genomic services Psomagen is offering? Psomagen, Inc is a fully operational, end-to-end multiomics service provider. Our specialties include comprehensive offering of Next Generation Sequencing such as WGS, WES, RNASeq, metagenomics, epigenomics, Sanger sequencing, and both RUO & CLIA/CAP NGS, as well as microarray interrogation, proteomic biomarker discovery, and bioinformatics. W ..read more

WHAT WORKFLOWS ARE AVAILABLE FOR COVID-19, INFLUENZA, AND RSV TESTING DURING THE 2022-2023 FLU SEASON? Rise in Flu & RSV Infections Public Health Institutions such as the US CDC are reporting increase in cases for several respiratory diseases such as RSV1 and Influenza 2. Additionally, health officials are warning that we could have a bad flu season this year3. PerkinElmer is equipped to provide customers with everything they need to provide their patients and clients with high quality equipment and test. Options for Different Reimbursement Strategies and Regions Multiplex EUA authorized ..read more

TIPS ON ESTABLISHING A RELIABLE CELL FREE DNA (cfDNA) WORKFLOW FROM PLASMA SAMPLES Analyzing circulating cell free DNA (ccfDNA) offers a non-invasive, dynamic window into the body’s physiological state and its use as a biomarker is gaining rapidly. However, multiple challenges remain with establishing its routine application. This is mainly due to its low levels, high rates of fragmentation, and preanalytical variabilities associated with sample collection, cfDNA extraction and quantification. Here, we go through some tips on how one can establish a reliable cfDNA workflow from plasma sampl ..read more

SURVEILLANCE PLANS ARE ESSENTIAL FOR PANDEMIC PREPAREDNESS AND PREVENTION The main lesson being learned from the COVID-19 pandemic is the importance of detecting cases early, following up on close contacts and preventing further disease spread for as long as possible. Other new and emerging diseases can affect us anywhere and at any time. The European Centre for Disease Prevention and Control, the World Health Organization Regional Office for Europe, and the European surveillance networks for COVID-19 and influenza agree on the urgent need to develop and sustain resilient population based i ..read more

MEETING IVDR REQUIREMENTS & MORE WITH NEW CE-IVD chemagic NUCLEIC ACID PURIFICATION KITS What is IVDR? In vitro diagnostic (IVD) manufacturers have been busy bees in the past months, working intensively to meet the new European Union regulations known as the European In Vitro Diagnostic Medical Device Regulation (EU IVDR) that come into play 26th May 2022. Under the EU IVDR which replaces the older In Vitro Diagnostic Medical Devices Directive (IVDD), a higher emphasis is placed on quality management systems and post-market surveillance, with more stringent conformity asse ..read more

THE NEW NORMAL: LESS EXPENSIVE, FASTER, COMPLETELY AUTOMATED NORMALIZATION OF NGS LIBRARIES Incorporating Normalization Beads into your NGS Workflow Normalization is the process by which NGS libraries of different concentrations are diluted to the same final concentration before pooling and sequencing. This step is needed to obtain best data quality from a run. Traditional normalization requires library quantification, calculations, and adjusting the concentration of each library by pipetting different volumes of buffer. Though normalization is crucial, the tradition protocol places time an ..read more

THE HUMAN ATLAS Expanding the Understanding of Human Health and Disease The international Human Cell Atlas (HCA) consortium was established to increase our understanding of human health and disease using single cell genomics and spatial imaging technologies to map every cell type in the human body. Data collected by the HCA is allowing scientists to identify and expand their understanding of cell types. Recently, four studies by members of the HCA consortium were published in Science (1-4). These studies reveled single-cell and single-nucleus transcriptome maps of more than 1 million human ce ..read more

AN INTRODUCTION TO SINGLE-CELL RNA SEQUENCING Hints from Honeycomb: How Does Single Cell RNA Sequencing Work? Article Author: Generating a comprehensive gene expression profile for a sample type of interest is essential for understanding its underlying biology. Two primary methods of RNA sequencing (RNAseq) are: Bulk RNAseq, which shows the gene expression for a sample as a whole, providing an average view of everything that is being transcribed in all of the cells. Single-cell RNAseq (scRNAseq), which allows researchers to determine the distinct gene expression profiles of individual c ..read more

ON DEMAND WEBINAR: EMPOWERING AGRIGENOMICS THROUGH TECHNOLOGY This workshop, hosted by PerkinElmer, explores innovative, reliable workflows developed to propel the efficiency of your genomics-based agricultural research workflows. Featured Speakers: Revolution Thru Automation Covers a four-year journey from zero automation to full automating of all library preparation, and then taking that to the next level moving from a 96 well format to 384 with full miniaturization for both DNA and RNA preps. Expanding the Reach of Single-Cell Profiling with the HIVE scRNAseq Solution The HIVE ..read more