Six-week-old mice were sensitised using a two-step method using Culicoides extract, and the model was evaluated by behavioural, histopathology and specific antibody detection. Immunohistochemical staining showed a significant increase in the infiltration of CD4 and CD8 T cells into the dermal tissue of Culicoides-sensitised mice, compared to control mice. Serum IgE (p < 0. 05) and IgG1 (p < 0. 05). In vitro stimulation with Culicoides extract induced significant proliferation of splenocytes (p < 0.001), and the mRNA expression of interleukin (IL)-4, IL-5, IL-10 and IL-13, which were ..read more

Lactic acid bacteria specifically Lactobacillus, abundantly present in cow milk, have demonstrated significant broad-spectrum antibacterial efficacy against multidrug-resistant bacteria, particularly through the expression of plantaricin (pln EF) coding genes. The occurrence of lactobacillus spp. was 72.89%, with the plnEF coding gene detected in 4.96%. Lactobacillus isolates carrying the plnEF gene exhibited significant inhibition zones against Salmonella Typhimurium (24 mm) and Salmonella enterica (24 mm), Escherichia coli (11 mm), Staphylococcus aureus (14 mm), Klebsiella pneumon ..read more

Methionine is often considered to be the first limiting amino acid in commercial broiler diets. Broiler diets are commonly supplemented with dl-methionine (dl-Met) and/or liquid methionine hydroxy analogue-free acid (MHA-FA), chemically known as 2-hydroxy-4-methylbutanoic acid, to meet the bird's sulphur amino acid requirements. The relative biological efficacies of liquid MHA-FA compared to dl-Met on an equimolar basis were estimated 66%–89% (59%–79% on a weight-to-weight basis). Abstract Background In the broiler's diets based on corn–soya bean meal, methionine (Met) and cystine (Cys), kno ..read more

Antibiotic residue builds up as a result of the continuous use of antibiotics like oxytetracycline or tetracycline to cure illnesses, prevent the spread of diseases, and promote animal growth. Residual accumulation in animal source food will lead to different adverse effects to humans and animals. The low dose exposure by microorganisms leads development of antimicrobial resistance. Abstract Background Antibiotics are commonly used drugs in farm animals for therapeutic, prophylactic and diagnosis purposes. As a result of the use of antibiotics in livestock, residues of antibiotics may be pre ..read more

This retrospective study aimed to investigate the prevalence and breed predisposition to feline respiratory diseases in Japan and determine the association between clinical signs, duration and type of respiratory diseases. Six hundred fifteen respiratory diagnoses were included, and some associations between popular breeds in Japan and respiratory diseases were observed. Furthermore, some associations between clinical signs, duration and type of respiratory diseases were also found. Abstract Background Few epidemiological studies on respiratory medicine and the relationship between clinical ..read more

This study investigated the effects of dietary nanoliposome coated astaxanthin (NA) on the haematological parameters, immune responses and antioxidant status of rainbow trout. Results showed that increasing levels of NA in the fish diet led to significant improvements in red blood cell count, haemoglobin concentration and white blood cell count, indicating enhanced haematological parameters. Additionally, the study demonstrated that NA positively influenced the antioxidant capacity and immune responses of the rainbow trout. Abstract Background Astaxanthin is the most prevalent carotenoid in ..read more

Using selected gut-related biomarkers, this study investigated whether there is an association between canine atopic dermatitis and intestinal damage. Increased intestinal biomarkers in dogs with atopic dermatitis may indicate the presence of intestinal damage. In addition, the development of atopic dermatitis may be predisposed to the entry of allergens into the body through sites of intestinal damage. Abstract Background A significant association between atopic dermatitis and leaky gut syndrome has been demonstrated in humans. No studies have been conducted to determine whether there is an ..read more

The results revealed that ABZSO2 was the main metabolite of ABZSO in donkey plasma. The concentrations of ABZSO and its cheif metabolite (ABZSO2) were detected in liver, kidney, skin and muscle; however, ABZ-SO2NH2 was only detected in liver and kidney. Abstract Background Various anti-parasitic drugs are used to control donkey parasitic diseases. The abuse of donkey drugs leads to the disposition of residues in the edible parts of treated donkeys. Objectives The aim of this study was to (1) analyse the pharmacokinetics of ABZSO to serve as reference for the dosage regimen in donkey; and (2 ..read more

This study evaluated the concentration of peritoneal fluid lactate concentration in systemically healthy mares in the first 36 h post-partum. The study determined the reference range for this parameter in this healthy population to be 0–2.5 mmol/L (mean bias +0.3 mmol/L, fold-increase = 1). This is similar to the reference range of peritoneal lactate concentration in the general population. Lactate concentration was also not influenced by concurrent retainment of foetal membranes in this population nor by age or breed. Abstract Background Peritoneal fluid lactate con ..read more

The subject of my article is the synthesis of chitosan nanoparticles in conjunction with the utilization of mupirocin and honey. Our aim was to examine the combined effect of these three substances on combating methicillin-resistant Staphylococcus aureus (MRSA), a strain known for its resistance to conventional treatments. Until now, the combined impact of these three substances had not been thoroughly investigated. Given the urgent issue of antibiotic resistance in today's world, the significance of utilizing nanoparticles and materials with antibacterial and antioxidant properties cannot be ..read more