Background: Pseudomonas aeruginosa is an opportunistic pathogen that represents a global public health threat. Immunocompromised hosts and cystic fibrosis patients are primarily susceptible to high morbidity and mortality due to pulmonary tract colonization with mucoid Pseudomonas aeruginosa. Differentially expressed virulence factors govern Pseudomonas aeruginosa pathogenicity in different sites of infection. Therefore, the detection of site-specific virulence genes in Pseudomonas aeruginosa isolates may potentially predict the level of Pseudomonas aeruginosa pathogenicity and the outcome in ..read more

Background: Development of multidrug-resistant (MDR) organisms is becoming a critical clinical problem for the management of numerous bacterial infections. Investigating other antibacterial drugs is urgently needed that are nontoxic and effective by different mechanisms. Methods: This study was aimed to develop silver nanoparticles (AgNPs) utilizing a green synthetic approach involving highly solubilized curcumin in freeze dried nanocrystals (CrNC) as a natural flavonoid reducing agent. Results: The fabricated silver nanoparticles were typically found to be spherical in shape with particle siz ..read more

Background: Uropathogens other than Escherichia coli can be used in combination with other chemotherapeutic agents due to genetic differences in the implicated uropathogens; hence, there is a focus on the microbiological qualities of homemade beverages. This study investigated the microorganisms present in homemade drinks (Kunnu, Soymilk, and Zobo) and their effects on common uropathogens (Escherichia coli, Staphylococcus aureus, Candida albicans, and Pseudomonas aeruginosa). Methods: Biochemical characterization, an antibiotic susceptibility test (AST) and a growth inhibition test were perfor ..read more

Background: The wide use of azoles for the prevention and treatment of candidiasis is due to the high morbidity and mortality associated with this nosocomial infection. Consequently, drug resistance has emerged among Candida species. The current study aimed to compare between the VITEK-2 and the chromogenic media as regards the identification of Candida species and antifungal susceptibility testing (AFST) using agar dilution of different antifungals with multiple concentrations. Methods: This cross-sectional study was conducted on 160 Candida isolates collected from different specimens submitt ..read more

Background: The global health challenge posed by malaria continues to be significant, with 249 million clinical cases documented in 2022, leading to more than 600,000 deaths, primarily among children living in endemic regions. Despite a decline in malaria mortality in the early 2000s, recent trends show a concerning rise in confirmed incidence, surpassing pre-COVID-19 pandemic levels. The imminent widespread introduction of two commercially available vaccines against Plasmodium falciparum marks a pivotal advancement in prevention of this major human pathogen, albeit with moderate conc ..read more

Back ground: Plants have been used for many generations for healing purposes, and screening of plant extracts have yielded positive results in the developments of clinically effective drugs. Objective: The present study aims to phytochemically investigate and biologically evaluate the antimicrobial potential of Gnidia glauca gilg root used to manage microbial infections in parts of Ethiopia. Martials and Methods: Root of Gnidia glauca gilg was collected from wollega University garden, Ethiopia. The collected plant material was cleaned to remove the dust particulate and the bark was carefully r ..read more

Background: Monkeypox disease caused by the monkeypox virus is a primary public concern worldwide, especially in developing countries. This virus contains several proteins thought to be responsible for the onset of monkeypox disease. Many of these proteins are hypothetical, and their functions have yet to be discovered. A hypothetical protein [Monkeypox virus] (accession number URK21226.1) was selected in this study for extensive structural and functional analysis. Our study will predict this protein's homological structure and biological function to acquire more knowledge about the protein. M ..read more

Background: Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection could be associated with other co/super-infections that worsen the outcome. In our hospital, the burden of viral co-infections with SARS-CoV-2 is unknown. We aimed to identify the lower respiratory tract viral pathogens causing co-infections among SARS-CoV-2 patients, and to elucidate their outcome. Subjects and Methods: We enrolled 147 patients with SARS-CoV-2 infection. Lower respiratory viral co-infection was identified in non-repetitive respiratory specimens by Biofire FilmArray Pneumonia Panel. Cul ..read more

Objective: The main goal of this current study was to assess the prevalence of these infections in the outpatient setting as well as in hospitalized patients at Mohammed V Hospital in Meknes, Morocco. Method:  The epidemiological and microbiological characteristics of respiratory infections mentioned in the registers of the laboratory of microbiology at Mohammed V Hospital, Meknes, were collected on pre-established exploitation sheets for each case throughout a 3-year period (2017-2019). Results:  A total of 7863 patients diagnosed with respiratory infection ..read more

Background: The rapid expansion of the aquaculture industry, particularly shrimp farming in developing Asian countries, has resulted in increased antibiotic usage. This indiscriminate use raises concerns about the selection of bacteria resistant to multiple antibiotics, potentially leading to environmental dissemination and posing risks to public health. This study investigates the occurrence and characteristics of antibiotic-resistant bacteria in shrimp from South India, highlighting the potential risks to public health. Methods: Shrimp samples were collected from t ..read more