BIODIVERSITY STUDY OF Zilla spinosa (L.) IN EGYPT
Egyptian Journal of Genetics And Cytology
by AMANY S. ABDO, SHAFIK D. IBRAHIM, MONA E. ABD EL-GAWAD
1M ago
Zilla is a monospecific genus in the flora of Egypt represented by Zilla spinosa. The field observations of Zilla spinosa populations showed the presence of a high degree of morphological diversity. The taxonomic problem of this species is mainly related to its confused infra-specific treatments in different floras. Our taxonomic revision of Z. spinosa was carried out on 15 recently collected populations covered the geographical range of this species in Egypt, in addition to 33 old herbarium specimens from different localities dating to nine decades ago. Herbarium specimens and recently obtain ..read more
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MOLECULAR PROFILING AND GENETIC DIVERSITY OF SHEEP POX AND LUMBY SKIN DISEASE VIRUS
Egyptian Journal of Genetics And Cytology
by MONA S.M. AWAD ALLAH, SAMER A. IBRAHIM, ASHRAF B. ABDEL RAZIK, SOAD M. SOLIMAN, KHALED A. METWALLY
1M ago
The objective of this research was to investigate the molecular genetic similarities between LSDV and SPV, which have spread extensively in Egypt. The aim was to identify these viruses through the propagation of LSDV on MDBK cells and SPV on VERO cells, as well as through PCR analysis and sequencing for G-protein-coupled chemokine receptor (GPCR) specific for LSDV and viral envelope protein gene (P32) specific for SPV of the isolated viruses. The obtained sequences were then compared using a phylogenetic tree to determine any immunogenic factors that could explain why cattle exhibit immunity w ..read more
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ESTABLISHMENT OF HIGHLY REPRODUCIBLE PROTOCOL OF HARMAL (PEGANUM HARMALA L.) THROUGH IN VITRO PROPAGATION
Egyptian Journal of Genetics And Cytology
by REEM S. EL-GHANNAM, GIHAN M. H. HUSSEIN, RABAB A. ABDULHAI, ZINAB A. ABDELGWAD
1M ago
Since ancient times, medicinal plants have been essential to human civilization, and they are still essential now. Peganum harmala L. regarded as one of the most significant medicinal plants. This perennial herb is indigenous to Central Asia and the Mediterranean region and is a member of the Nitraraiceae family. Peganum harmala L. is widely distributed in Egypt along the left bank of the Mediterranean, in Sinai, the Eastern Desert, and in open areas with disturbed ground. Recent reports indicate that this plant contain beta-carbolines alkaloids such as harmaline, harmine and harmalol therefor ..read more
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EFFICACY OF TITANIUM DIOXIDE NANOPARTICLES IN BANANA MICROPROPAGATION AND THEIR EFFECT ON DNA CHANGE
Egyptian Journal of Genetics And Cytology
by SAMAH M. M. ELDEMERY, SAMAH S. NOORELDEEN, ASMAA M. ZAKARIA, EBTSAM M. HAMZA, KAMAL F. ABDELLATIF
6M ago
Titanium dioxide nanoparticles (TiO2NPs) minimize contamination and enhance proliferation and multiplication of banana through micropropagation. The effect of TiO2NPs on banana tissue culture (micropropagation cultivar Grand Nain) as well as on banana DNA was studied using different concentrations (i.e., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20 and 30 mg/L). DNA was extracted from the regenerated plantlets along with the normal plant. TiO2NPs (5 mg/L) decreased the contamination and browning percentage of banana cultures to the minimum degree concentration (0%). In addition, all micropropagation trai ..read more
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DETERMINATION OFJOJOBA (Simmondsia chinensis) PLANT GENDER BASED ON A MALE-SPECIFIC DNA FRAGMENTS USING PCR ASSAY
Egyptian Journal of Genetics And Cytology
by SHAFIK D. IBRAHIM, TAMER A. EL-AKKAD, FAYROUZ A. NOSSIR, SHAIMAA M. AHMED
6M ago
Agricultural production in desert and semi-arid regions is limited due to harsh conditions, however, advances in biotechnology offer new opportunities. One promising crop is jojoba, which requires a specific male and female planting ratio for optimal growth. Therefore, early identification of the plants' gender is crucial for successful cultivation. This study developed PCR primers generated from male-specific DNA sequences that distinguish between male and female jojoba plants. One set of primers, HQF and HQR produced a distinct fragment (~281 bp) in male plant samples only. Also, KP76F and K ..read more
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CORRELATION BETWEEN CIRCULATING CYTOTOXIC MARKERS (CD3 & CD8) AND DISEASE SEVERITY IN PNEUMONIA COVID-19 PATIENTS
Egyptian Journal of Genetics And Cytology
by MARWA M. RADWAN, HALA G. METAWALY, M. I. NASR, GHADA M. NASR
7M ago
Among common studies, C- C-reactive protein has been thought to be the most reliable predictor of severity and death in COVID-19 patients. According to the current findings, COVID-19 patients' severity and death were substantially linked with elevated CRP levels. Additionally, prognosis, outcome, and mortality may be predicted using the dynamic assessments of CD3+, CD8+, LDH, ferritin, AST, ALT, and lymphocytes in COVID-19 patients. These inflammatory biomarkers and clinical traits require extensive additional study. Conclusion: CD8+ T cells can be for diagnosing COVID-19 cases. CRP levels ros ..read more
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ESTIMATION OF NITRIC OXIDE SYNTHASE EXPRESSION LEVEL IN DISTINGUISHING PSORIASIS FROM ATOPIC DERMATITIS AND CORRELATING IT WITH DISEASE SEVERITY
Egyptian Journal of Genetics And Cytology
by MANAL O. EL HAMSHARY, AYMAN M. ELREFAEI, HISHAM A. ISMAIL, AZZA G. FARAG, MAHMOUD I. NASR, GHADA M. NASR
11M ago
NOS2 may play a role in the distinction between psoriasis and eczema. NOS2 expression was upregulated in psoriasis compared to eczema, which means that psoriasis could be routinely differentially diagnosed with high sensitivity and specificity by measuring NOS2 expression level ..read more
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PHYLOGENETIC ANALYSIS OF EGYPTIAN FABA BEAN CULTIVARS (Vicia faba) AS REVEALED BY SRAP AND SSR MARKERS
Egyptian Journal of Genetics And Cytology
by KHALID S. ABDEL-LATEIF, ENAS M. ABD EL-GHANY
11M ago
SRAP and SSR markers were employed efficiently to discover the genetic distinction and phylogenetic relationships among five cultivars of Egyptian Faba bean (Giza 716, Giza 843, Sakha 4, Mariot 2 and Nubaria 5). SRAP primers exhibited a total of 70 bands and 35 (50%) were polymorphic bands. All the SSR markers exhibited clear bands; five SSR markers (KVFG460, KVFT1400, KVFT5761, KVFT906, and KVFT1913) gave two bands, while KVFG588 marker produced only one band. The tested SSR markers produced eleven bands, whereas 27 % of these bands were polymorphic. Each of SRAP and SSR markers established s ..read more
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PHYLOGENETIC ANALYSIS OF EGYPTIAN FABA BEAN CULTIVARS (Vicia faba) AS REVEALED BY SRAP AND SSR MARKERS
Egyptian Journal of Genetics And Cytology
by KHALID S. ABDEL-LATEIF, ENAS M. ABD EL-GHANY
1y ago
SRAP and SSR markers were employed efficiently to discover the genetic distinction and phylogenetic relationships among five cultivars of Egyptian Faba bean (Giza 716, Giza 843, Sakha 4, Mariot 2 and Nubaria 5). SRAP primers exhibited a total of 70 bands and 35 (50%) were polymorphic bands. All the SSR markers exhibited clear bands; five SSR markers (KVFG460, KVFT1400, KVFT5761, KVFT906, and KVFT1913) gave two bands, while KVFG588 marker produced only one band. The tested SSR markers produced eleven bands, whereas 27 % of these bands were polymorphic. Each of SRAP and SSR markers established s ..read more
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EVALUATION OF SALINITY TOLERANCE IN SOME BREAD WHEAT RECOMBINANT INBRED LINES USING MICROSATELLITES MARKERS
Egyptian Journal of Genetics And Cytology
by AYA-TU-ALLAH E. A. MANCY, F.M. ABDEL-TAWAB, A. H. ABODOMA, MAHMOUD MAGDY, THARWAT M. ELAMEEN
1y ago
In most countries worldwide, including Egypt, bread wheat is essential among cereals crops. However, soil salinity is a global issue that has a negative impact on plant growth, development, and productivity. Therefore, salt tolerance is an important feature that must be improved in wheat genotypes. Identifying informative and highly differential molecular markers is critical for developing salt-tolerant genotypes that could tolerate excessive salts in the soil. Twelve bread wheat recombinant inbred lines (RILs) derived from a cross between Shandaweel-1 and Giza-168, were evaluated in pots foll ..read more
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