Abstract Scope Bile acids (BAs) have recently emerged as important regulators of many physiological and pathological processes. However, the change of colonic BAs induced by high-fat diet (HFD) and their effects on colonic barrier function remain to be further elucidated. Methods and results C57BL/6 mice were divided into two groups and feed 12 weeks with diets differing for fat content. Higher levels of serum DAO activity, ET and D-LA were observed in HFD-fed mice, indicating an increase in intestinal permeability. Real-time quantitative PCR and western blot analyses demonstrated that HFD dow ..read more

Abstract Lactic acid fermentation modulates the composition of food, leading to changes in safety, sensory and nutritional properties. The effects of lactic fermented pineapple juice supplementation on energetic metabolism of high-fat diet (HFD) fed mice were compared with either water (control), sweetened water, bacteria in sweetened water and pineapple juice supplementation. Drink consumption and body weight were measured during the six weeks of experiment, whereas glycaemia and lipid content were determined at the beginning and at the end of the experiment. Total energy intake was similar b ..read more

Mol. Nutr. Food Res. 2023, 67, 202200529 DOI: 10.1002/mnfr.202200221 Production conditions play a role in determining the probiotic properties of Streptococcus thermophilus. During the manufacturing process of the probiotic S. thermophilus BT01, the use of an appropriate cryoprotectant led to a significant reduction in fecal urease activity in healthy subjects during a randomized controlled crossover study. This is reported by Anđela Martinović, Marco Chittaro, Diego Mora, and Stefania Arioli in article number 2200529 ..read more

Molecular Nutrition &Food Research, Volume 67, Issue 6, March 2023 ..read more

Abstract Scope Holothuria leucospilota polysaccharides (HLP) are bioactive polysaccharides with immunomodulatory effects. This study aimed to investigate the impact of HLP on dextran sodium sulfate (DSS)-induced colitis in rats and further investigate the complex interactions between changes in intestinal microbiota, co-metabolites, and intestinal inflammation under HLP intervention. Methods and Results The ulcerative colitis (UC) model of Sprague Dawley (SD) rats was established by a normal diet with 3%DSS. The effects of HLP on UC were studied by gavage of different doses of HLP for two week ..read more

Abstract Scope Fucosylated human milk oligosaccharides (fHMOs) are metabolized by Bifidobacterium infantis and promote syntrophic interactions between microbiota that colonize the infant gut. The role of fHMO structure on syntrophic interactions and net microbiome function is not yet fully understood. Methods and Results Metabolite production and microbial populations were tracked during mono- and co-culture fermentations of 2ʹfucosyllactose (2ʹFL) and difucosyllactose (DFL) by two B. infantis strains and Eubacterium hallii. This was also conducted in an in vitro modeled microbiome supplemente ..read more

Abstract Scope Colonic mucosal healing is the terminal goal for the treatment of ulcerative colitis (UC), but there is currently no specific drug available. This study investigated the beneficial effect of diallyl trisulfide (DATS) on the colonic mucosal healing. Methods and Results Dextran sulfate sodium (DSS) was used to induce colitis in female C57BL/6 mice, and DATS was orally administered during the recovery period. DATS hardly impacted the inflammation of the colonic tissues, but significantly promoted the mucosal repair. DATS promoted the migration but not proliferation of colonic epith ..read more

Abstract Scope : Two peptides VY (Val-Tyr) and SFLLR (Ser-Phe-Leu-Leu-Arg) were recently identified from soy-fermented douchi with hypoglycemic activity in cells. The study aimed to understand their potential effects on glucose metabolism and insulin sensitivity as well as their mechanisms of action in a high-fat diet (HFD) induced insulin resistant model. Methods and results : C57BL/6 mice were fed HFD for 8 weeks, followed by peptide supplementation (doses: 10 and 50 mg/kg body weight) for 8 weeks. Peptides supplementation, especially SFLLR, reduced body weight gain, insulin resistance, hype ..read more

Abstract Scope Human milk (HM) has a wide range of proteins with biological and nutritional functions, essential for newborns. The roles of proteins and their proteoforms in HM are not fully understood. This study aimed to assess, by 2-DE proteomics, the differential proteoforms in HM, present in colostrum (COL), transition (TRA) and mature milk (MAT), aiming to contribute to understanding neonates' protein needs. Methods and Results HM samples were collected from 39 healthy lactating women. COL presented the higher concentration of essential amino acids. After MALDI-MS/MS and bioinformatics a ..read more

Abstract Scope Bile acid (BA) dysregulations are closely associated with non-alcoholic fatty liver disease (NAFLD) in both children and adults. Most of the previous clinical and animal studies investigated BA disturbances in the blood or feces samples. How BAs change within the enterohepatic circulation during the onset and progression of NAFLD as biomarkers deserves to be explored. Methods and Results Four-week-old male mice were fed with a high-fat diet plus 4% w/v fructose drinking water (HFF) or chow diet with tap water (ND) for 4 and 12 weeks, correspondingly. In comparison, eight-week-ol ..read more