Oocytes of KPNA2 KO mice can be normally fertilized and undergo cleavage into 2cell embryos where their development arrests. One reason for this arrest is a disturbed translocation of NPM2 from the cytosol into the nucleus in zygotes and 2cell embryos. Although several other KPNA paralogs are expressed from the maternal genome at this embryonic stage, NPM2 binds primarily to KPNA2. Thus, the absence of KPNA2 directly affects the nuclear import of NPM2 leading to defective activation of the embryonic genome. Abstract The nuclear transport of proteins plays an important role in mediating the t ..read more

PKCε and MEG2 competitively bind to STAT3, regulating IL-6-mediated GLUT3 and TREM2 during inflammatory pain. PKCε, MEG2, and STAT3 activation engaged with IL-6-mediated microglial GLUT3 and TREM2 during inflammatory nociceptive transmission. Under stimulation, upregulated PKCε and MEG2 competitively bind to phosphorylated STAT3 (especially, the Ser727 binding site). PKCε is dominant over MEG2 in STAT3 binding assay, subsequently increasing IL-6 promoter activity, manifested as IL-6 cytokine-mediated microglial hyperalgesia during inflammatory pain. PKCε, protein kinase C epsilon; MEG2, megak ..read more

The FASEB Journal, Volume 38, Issue 8, 30 April 2024 ..read more

Phosphoenolpyruvate kinase 1 (Pck1) increased in intestinal epithelial cells (IECs) during AP. Inhibition of Pck1 by 3-Mercaptopicolinic acid hydrochloride (3-MPA) in AP mice alleviated pancreatic and ileal injuries, reduced intestinal permeability and bacteria translocation, restored Paneth cells and goblet cell function, and improved intestinal immune response, including reduction in neutrophils infiltration and elevation of M2/M1 macrophages ratio and secretory immunoglobulin A (sIgA). These findings indicated that inhibition of Pck1 in IECs might alleviate AP via modulating intestinal hom ..read more

Repeated sprint training in normoxia and hypoxia increased exercise performance and affected skeletal muscle metabolism. The levels of proteins involved in the oxidative phosphorylation pathway were decreased, while proteins involved in the glycolytic pathway were upregulated after both training modalities. Repeated sprint training in hypoxia potentiated the increase in the glycolytic phenotype likely through the activation of a pathway involving S100A13 and the Akt axis. Created with BioRender.com. Abstract Athletes increasingly engage in repeated sprint training consisting in repeated shor ..read more

Myofiber denervation and senescent cell accumulation are hallmarks of skeletal muscle aging. We investigated the effects of unilateral leg extensor resistance training (8 weeks, 2 days/week) on denervated myofibers, senescent cells, and associated protein markers in middle-aged participants (MA, 55 ± 8 years old, 17 females, 9 males). Training significantly increased satellite cell number. However, despite affecting the expression of proteins associated with denervation and senescence, training did not alter denervated myofibers, senescent cells, or most assayed senescence-associated secretor ..read more

A novel knock-in mouse model has been generated expressing a rhodopsin mutant prone to mislocalization. This model has enabled us to analyze alterations in the retinal proteome that affect the transport of rhodopsin and other proteins toward the photoreceptor sensory cilia. Abstract Rhodopsin mislocalization encompasses various blind conditions. Rhodopsin mislocalization is the primary factor leading to rod photoreceptor dysfunction and degeneration in autosomal dominant retinitis pigmentosa (adRP) caused by class I mutations. In this study, we report a new knock-in mouse model that harbors ..read more

Chronic opioid treatment disrupts intestinal epithelial barrier function by reducing antimicrobial peptides, thus causing an expansion of the mucin-degrading, Akkermansia muciniphila, “leaky” epithelium and inflammation. This contributes to the development of antinociceptive tolerance. Oral administration of butyrate or FMT prevents opioid-induced epithelial barrier disruption by increasing antimicrobial activity and reducing epithelial permeability and A. muciniphila. Improved epithelial barrier function results in a significant decrease in antinociceptive tolerance. Abstract Recent evidenc ..read more

The FASEB Journal, Volume 38, Issue 8, 30 April 2024 ..read more

During alveolar repair, alveolar type 2 (AT2) cell achieves self-renewal and differentiation via various transcription factors, signaling pathways, and biological processes. Besides, platelet-derived growth factor receptor alpha+ (PDGFRα+) lipofibroblasts, interstitial cells, macrophages, and angiocrine support the growth and differentiation of AT2 cells. HOP homeobox+ (HOPX+) alveolar type 1 (AT1) cells and airway epithelial cells have the capacity to differentiate into AT2 or AT1 cells. Abstract Considerable progress has been made in understanding the function of alveolar epithelial cells ..read more