8:3. DNA Libraries and Generating cDNA
MCAT Modules | Review
by Hamza Taj
1y ago
Prot —> mRNA —> cDNA —> dsDNA. (Step 2 via reverse transcriptase and step 3 via DNA Polymerase). Now the dsDNA is injected into a virus which infects bacteria to make lots of the DNA ..read more
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8:2. Polymerase Chain Reaction
MCAT Modules | Review
by Hamza Taj
1y ago
Denaturation (Heat to high temp), Primer Annealing (Cool slightly), Primer Extension via polymerase (Heat but not as much as step 1 ..read more
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8:1. Gel Electrophoresis
MCAT Modules | Review
by Hamza Taj
1y ago
GE find us the length of a DNA Strand (how many base pairs long ..read more
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7:5. Applying the HP Equation
MCAT Modules | Review
by Hamza Taj
1y ago
Not really sure why I did a module on this - basically you know they’re homo recessive because they have blue eyes (the recessive trait), and you know to use q BECAUSE we’re dealing with the recessive trait ..read more
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7:4. The Hardy-Weinberg Equation
MCAT Modules | Review
by Hamza Taj
1y ago
HP Equation gives P(allele frequencies). P^2 is probability of homo dominant, Q^2 is probability of homo recessive, 2pq is probability of heterozygous allele combo. P = frequency of dom allele, Q = frequency of recessive allele ..read more
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7:3. Worked Example: Punnett Squares
MCAT Modules | Review
by Hamza Taj
1y ago
Discusses Dihybrid Crosses and the Law of Independent Assortment ..read more
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7:2. Co-dominance and Incomplete Dominance
MCAT Modules | Review
by Hamza Taj
1y ago
Don’t let the title fool you. This is short. Discusses complete, incomplete, and co-dominance ..read more
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7:1. An Introduction to Mendelian Genetics
MCAT Modules | Review
by Hamza Taj
1y ago
Discuss genes, alleles, geno/phenotypes, and punnet squares. The Khan Academy video discusses the history (Gregor Mendel) but let’s be real, who cares ..read more
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6:5. The Effects of Mutations
MCAT Modules | Review
by Hamza Taj
1y ago
Mutations can be good, bad, or both ..read more
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6:4. Mutagens and Carcinogens
MCAT Modules | Review
by Hamza Taj
1y ago
Define mutagens and then discuss endogenous and exogenous mutagens. (Endo: ROS); (Exo: intercalators, base analogues, carcinogens ..read more
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