Obtaining highly precise, accurate measurements is essential to experimental work in any field of science, molecular biology being no exception. The importance of reliable, accurate quantitation methods cannot be overstated, as these methods are crucial for conducting reproducible experiments. This is particularly relevant for the quantitation of proteins, which are studied in many different molecular biology labs due to their biological and clinical importance. As with many biological molecules, proteins are commonly quantified using some application of spectroscopy. However, due to the numer ..read more

One of the key aspects of viral protein research is the study of protein domains that remain mostly conserved across many different virus particles. These protein domains are critical for the virus’ propagation and survival, regulating important functions such as host recognition and genome insertion. One such domain is the ACE2 receptor binding domain, critical to the structure of coronavirus spike protein, which rose to public prominence after the COVID-19 pandemic. Spike proteins assemble into trimers on the surface of the mature virion and are found in all coronaviruses. Spike protein trim ..read more

Overview The ability to synthesize proteins using recombinant DNA techniques has revolutionized molecular and cell biology research by enabling the study of target proteins in a laboratory setting. Most commonly, this is done by transforming live cells with a plasmid that contains the gene for the protein of interest and transcriptional activators. The affordability and high yields this method produces make it a desirable first choice for those seeking to study a particular protein of interest. However, in vivo protein expression is very laborious and time-consuming, often taking days to weeks ..read more

ӒKTA Protein Purification Systems The popularization of methods to obtain large quantities of proteins, peptides, and other large biomolecules has led to the advent of fast protein liquid chromatography (also known as fast-performance liquid chromatography or medium pressure liquid chromatography). FPLC utilizes a peristaltic pump to accurately control the speed at which the protein sample (mobile phase) passes through the chromatography column of choice (solid phase). Unlike other chromatography techniques like high-pressure liquid chromatography (HPLC), FPLC operates at a much lower pressure ..read more

Determining the Optimal Temperature of Protein Expression Without any doubt, one of the greatest advances in molecular biology is the development of simple and easy to carry out protocols for the expression of proteins from Escherichia coli cells transformed by a plasmid. These plasmids are synthesized in the lab and typically contain three major regions of interest: an antibiotic resistance marker (for selection of successfully transformed cells), a lacZ promoter (for activation of a gene of interest upon addition of lactose), and a gene corresponding to the protein of interest. While other c ..read more

ARVYS Proteins Inc. provides a full spectrum of protein biochemistry services – recombinant protein expression in bacterial, insect and mammalian cells, protein purification, refolding, assays and assay development, protein characterization, fermentation and endotoxin removal. Outsource your protein biochemistry projects to ARVYS and enjoy superior results, team expertise and customer support after project completion. Affiliation – Caroline Goussen, Laëtitia Goldstein, Corinne Brèque, Bruno You, Stéphane Boyer,Damien Bataille, Ludovic Burlot LFB Biotechnologies, Direction Générale du Développe ..read more

ARVYS Proteins Inc. provides a full spectrum of protein biochemistry services – recombinant protein expression in bacterial, insect and mammalian cells, protein purification, refolding, assays and assay development, protein characterization, fermentation and endotoxin removal. Outsource your protein biochemistry projects to ARVYS and enjoy superior results, team expertise and customer support after project completion. Affiliation – Stockholm Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, The Arrhenius Laboratories for Natural Sciences, Stockho ..read more

ARVYS Proteins Inc. provides a full spectrum of protein biochemistry services – recombinant protein expression in bacterial, insect and mammalian cells, protein purification, refolding, assays and assay development, protein characterization, fermentation and endotoxin removal. Outsource your protein biochemistry projects to ARVYS and enjoy superior results, team expertise and customer support after project completion. Affiliations Oxford Expression Technologies Ltd, Bioinnovation Hub, Oxford, UK. Department of Biological Medical Sciences, Oxford Brookes University, Oxford, UK. Abstract Over ..read more

ARVYS Proteins Inc. provides a full spectrum of protein biochemistry services – recombinant protein expression in bacterial, insect and mammalian cells, protein purification, refolding, assays and assay development, protein characterization, fermentation and endotoxin removal. Outsource your protein biochemistry projects to ARVYS and enjoy superior results, team expertise and customer support after project completion. Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607, USA and 2Institut National de Recherche en Informatique et en Automatique, Paris 75012, France ..read more

ARVYS Proteins Inc. provides a full spectrum of protein biochemistry services – recombinant protein expression in bacterial, insect and mammalian cells, protein purification, refolding, assays and assay development, protein characterization, fermentation and endotoxin removal. Outsource your protein biochemistry projects to ARVYS and enjoy superior results, team expertise and customer support after project completion. Today we look at the publication from Humana Press.  Series Editor John M. Walker
School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL ..read more