Check it out: Researchers at the  [AxioMx, Abbratech Inc, Branford, CT, USA.] have developed a surrogate protein (‘MILKSHAKE’) using modified maltose binding protein (modMBP) and a target protein peptide containing a modified or nonmodified residue of interest for use in Western blot. Read the full article here:https://www.future-science.com/doi/10.2144/btn-2021-0078 Credit: Jones, K. S., Chapman, et al. Milkshake: Novel validation method for antibodies to post-translationally modified targets by surrogate western blot. BioTechniques, 72(1), 11–20, (2022). https://doi.org/10.2144/btn-202 ..read more

How to Store Western Blot PVDF membrane after transfer? Western blot membranes may need to be stored for future analysis or retesting. One advantage of polyvinylidene fluoride (PVDF) compared to nitrocellulose (NC) membranes is the stability of PVDF, a chemically resistant polymer with excellent long-term stability. Therefore, PVDF membranes can be stored and sample proteins can be preserved for long periods of time. Looking for PVDF Membranes for lab? NuSep PVDF Membrane is ideal for chemiluminescent and colorimetric western blots: it retains target protein very strongly during pro ..read more

The Transfer Process During Electrophoresis: Nitrocellulose(NC) Membranes   What is NC Membrane? Nitrocellulose membranes are a popular matrix used in protein blotting because of their high protein-binding affinity, compatibility with a variety of detection methods, and the ability to immobilize proteins, glycoproteins, or nucleic acids. The nitrocellulose membranes are pure nitrocellulose media that has a high binding capacity for proteins. It is easily wetted in water or transfer buffer and compatible with a wide range of protein detection systems. Compared to other types of membra ..read more

Transfer Basics The Importance Uses of Transfer Membrane Protein Transfer on to a Membrane Protein transfer is a vital step in western blot analysis which involves the transfer of proteins separated in a gel by electrophoresis to a solid support matrix. Immobilizing the protein to a solid support matrix facilitates the detection of specific proteins using antibodies directed against the protein(s) of interest. Typical solid matrices are membrane sheets of nitrocellulose and PVDF. Blotting After separating the protein mixture, it is transferred to a membrane. The transfer is done using an ele ..read more

The Transfer Process During Electrophoresis: Nitrocellulose(NC) Membranes   What is NC Membrane? Nitrocellulose membranes are a popular matrix used in protein blotting because of their high protein-binding affinity, compatibility with a variety of detection methods, and the ability to immobilize proteins, glycoproteins, or nucleic acids. The nitrocellulose membranes are pure nitrocellulose media that has a high binding capacity for proteins. It is easily wetted in water or transfer buffer and compatible with a wide range of protein detection systems. Compared to other types of membra ..read more

Transfer Basics The Importance Uses of Transfer Membrane Protein Transfer on to a Membrane Protein transfer is a vital step in western blot analysis which involves the transfer of proteins separated in a gel by electrophoresis to a solid support matrix. Immobilizing the protein to a solid support matrix facilitates the detection of specific proteins using antibodies directed against the protein(s) of interest. Typical solid matrices are membrane sheets of nitrocellulose and PVDF. Blotting After separating the protein mixture, it is transferred to a membrane. The transfer is done using an ele ..read more

Transfer Basics Protein Transfer on to a Membrane Protein transfer is a vital step in western blot analysis which involves the transfer of proteins separated in a gel by electrophoresis to a solid support matrix. Immobilizing the protein to a solid support matrix facilitates the detection of specific proteins using antibodies directed against the protein(s) of interest. Typical solid matrices are membrane sheets of nitrocellulose and PVDF. Blotting After separating the protein mixture, it is transferred to a membrane. The transfer is done using an electric field oriented perpendicular to t ..read more

The post Common Problems in Protein Electrophoresis Part II appeared first on NuSep ..read more

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The post Common Mistakes in Gel Electrophoresis appeared first on NuSep ..read more