Despite the advances in modern cancer therapy, malignant diseases are still a leading cause of morbidity and mortality worldwide. Conventional treatment methods frequently lead to side effects and drug resistance in patients, highlighting the need for novel therapeutic approaches. Recent findings have identified the existence of non-canonical micropeptides, an additional layer of the proteome complexity, also called the microproteome. These small peptides are a promising class of therapeutic agents with the potential to address the limitations of current cancer treatments. The microproteome is ..read more

Soluble interleukin 1 receptor-like 1 (ST2) is a circulating protein demonstrated to be associated with cardiovascular diseases; however, it has not been studied as a biomarker for peripheral artery disease (PAD). Using a prospectively recruited cohort of 476 patients (312 with PAD and 164 without PAD), we conducted a prognostic study of PAD using clinical/biomarker data. Plasma concentrations of three circulating proteins [ST2, cytokine-responsive gene-2 (CRG-2), vascular endothelial growth factor (VEGF)] were measured at baseline and the cohort was followed for 2 years. The outcome of intere ..read more

Chronic obstructive pulmonary disease (COPD) ranks as the third leading cause of global illness and mortality. It is commonly triggered by exposure to respiratory irritants like cigarette smoke or biofuel pollutants. This multifaceted condition manifests through an array of symptoms and lung irregularities, characterized by chronic inflammation and reduced lung function. Present therapies primarily rely on maintenance medications to alleviate symptoms, but fall short in impeding disease advancement. COPD’s diverse nature, influenced by various phenotypes, complicates diagnosis, neces ..read more

Capsular contracture (CC) is one of the most common postoperative complications associated with breast implant-associated infections. The mechanisms that lead to CC remain poorly understood. Plasma is an ideal biospecimen for early proteomics biomarker discovery. However, as high-abundance proteins mask signals from low-abundance proteins, identifying novel or specific proteins as biomarkers for a particular disease has been hampered. Here, we employed depletion of high-abundance plasma proteins followed by Tandem Mass Tag (TMT)-based quantitative proteomics to compare 10 healthy control patie ..read more

Proteomes, Vol. 12, Pages 21: Quantitative Analysis of Complement Membrane Attack Complex Proteins Associated with Extracellular Vesicles Proteomes doi: 10.3390/proteomes12030021 Authors: Illarion V. Turko Extracellular vesicles (EVs) represent a universal mechanism of intercellular communication in normal and pathological conditions. There are reports showing the presence of complement proteins in EV preparations, specifically those that can form a membrane attack complex (MAC). In the present work, we have used a quantitative mass spectrometry method that allows for the measurement of multip ..read more

Proteomes, Vol. 12, Pages 18: Investigating and Annotating the Human Peptidome Profile from Urine under Normal Physiological Conditions Proteomes doi: 10.3390/proteomes12030018 Authors: Amr Elguoshy Keiko Yamamoto Yoshitoshi Hirao Tomohiro Uchimoto Kengo Yanagita Tadashi Yamamoto Examining the composition of the typical urinary peptidome and identifying the enzymes responsible for its formation holds significant importance, as it mirrors the normal physiological state of the human body. Any deviation from this normal profile could serve as an indicator of pathological processes occurring in vi ..read more

Proteomes, Vol. 12, Pages 17: The Current State of Proteomics and Metabolomics for Inner Ear Health and Disease Proteomes doi: 10.3390/proteomes12020017 Authors: Motahare Khorrami Christopher Pastras Paul A. Haynes Mehdi Mirzaei Mohsen Asadnia Characterising inner ear disorders represents a significant challenge due to a lack of reliable experimental procedures and identified biomarkers. It is also difficult to access the complex microenvironments of the inner ear and investigate specific pathological indicators through conventional techniques. Omics technologies have the potential to play a v ..read more

Proteomes, Vol. 12, Pages 16: Plasma and Kidney Proteome Profiling Combined with Laser Capture Microdissection Reveal Large Increases in Immunoglobulins with Age Proteomes doi: 10.3390/proteomes12020016 Authors: Leanne J. G. Chan Niclas Olsson Magdalena Preciado López Kayley Hake Haruna Tomono Matthew A. Veras Fiona E. McAllister One of the main hallmarks of aging is aging-associated inflammation, also known as inflammaging. In this study, by comparing plasma and kidney proteome profiling of young and old mice using LC–MS profiling, we discovered that immunoglobulins are the prot ..read more

Proteomes, Vol. 12, Pages 15: Puzzle of Proteoform Variety—Where Is a Key? Proteomes doi: 10.3390/proteomes12020015 Authors: Stanislav Naryzhny One of the human proteome puzzles is an imbalance between the theoretically calculated and experimentally measured amounts of proteoforms. Considering the possibility of combinations of different post-translational modifications (PTMs), the quantity of possible proteoforms is huge. An estimation gives more than a million different proteoforms in each cell type. But, it seems that there is strict control over the production and maintenance of ..read more

Proteomes, Vol. 12, Pages 13: Key Proteomics Tools for Fundamental and Applied Microalgal Research Proteomes doi: 10.3390/proteomes12020013 Authors: Maxence Plouviez Eric Dubreucq Microscopic, photosynthetic prokaryotes and eukaryotes, collectively referred to as microalgae, are widely studied to improve our understanding of key metabolic pathways (e.g., photosynthesis) and for the development of biotechnological applications. Omics technologies, which are now common tools in biological research, have been shown to be critical in microalgal research. In the past decade, significant technologic ..read more