LINC00520 promotes colorectal cancer progression through miRNA-195-3p / NAT2 axis
Cellular and Molecular Biology
by Haibin Liu, Guochao Zhang, Chaofeng Li, Xin Song
1M ago
In this study, we investigated the role of LINC00520 in colorectal cancer (CRC) progression. We analyzed LINC00520 expression in 15 pairs of CRC tissues and adjacent tissues using qRT-PCR, revealing significantly elevated levels in CRC tissues and cell lines. Lentivirus-mediated up/down-regulation of LINC00520 in CRC cell lines demonstrated that increased LINC00520 expression enhanced cell invasiveness, as confirmed by transwell and wound healing assays. Bioinformatics analysis identified a regulatory axis involving LINC00520, microRNA-195-3p, and NAT2. Luciferase assays confirmed direct bindi ..read more
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In vivo and in vitro studies on the role of regulating Smac expression in the occurrence and development of colon cancer
Cellular and Molecular Biology
by Donglei Zhang, Ning Yang
1M ago
We aimed to explore the role of regulating Smac expression levels in the occurrence and development of colon cancer through in vitro and in vivo experiments. Colon cancer cells HT-29 were cultured and transfected into different groups. qRT-PCR was used to detect the expression level of Smac in cells; Flow cytometry was used to detect the apoptotic ability of each group of cells; Western blot was used to detect the protein expression of Smac and apoptosis-related factors Survivin and Caspase-3; The nude mouse tumorigenesis experiment was conducted to detect the regulatory effect of regulating S ..read more
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The effect of vitamin E incorporated into injectable platelet-rich fibrin on orthodontic tooth movement in rabbits
Cellular and Molecular Biology
by Mahmoud Kanan Mohsin, Zana Qadir Omer
1M ago
To evaluate the effects of injectable platelet fibrin (iPRF) and combined vitamin E-iPRF on orthodontic tooth movement (OTM) rates in rabbits, 35 male New Zealand white rabbits were involved in this study using splitmouth design. OTM was carried out on the mandibular first premolar using 100g nickel titanium closing coil. Right side served as study group, isolated iPRF in one group and combined vitamin E-iPRF in other group was injected buccally and lingually (iPRF group, Vit E-iPRF group), and left side acted as positive control group (CG) by injecting normal saline (positive CG). The rate of ..read more
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The efficacy of Bortezomib-based regimens on survival state in newly diagnosed multiple myeloma patients
Cellular and Molecular Biology
by Kawa Muhamedamin Hasan
1M ago
The prognosis of patients with multiple myeloma (MM) has significantly improved over the past ten years because of several innovative treatments, including the proteasome inhibitor Bortezomib and immunomodulatory drugs (IMiDs) like Thalidomide and Lenalidomide. The present study aimed to determine the effectiveness of Bortezomib-based regimens on survival state of MM patients. This retrospective study included 204 newly diagnosed MM patients who were registered at Nanakali Hospital for Blood Diseases and Cancer, Erbil- Iraq, between April 2008 and April 2022. The patients were split into two p ..read more
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MicroR-1199-5p targeting SRD5A2 promotes the biological behavior and EMT of hypospadias cells
Cellular and Molecular Biology
by Yifu Chen, Jianjun Hu, Liucheng Peng, Yaowang Zhao
1M ago
Hypospadias, an oft-occurring penis anomaly, ranks among neonatal's foremost birth defects. The SRD5A2 can affect male reproductive system development and is abnormally expressed in its epithelial cells. This study exploration aimed at understanding the role of SRD5A2 in the development of hypospadias from a molecular perspective. SRD5A2 levels in hypospadias primary cells were analyzed by Western blot, while targeted interaction with miR-1199-5p was ascertained by dual-luciferase gene reporter assay. In vitro biological experiments were used to confirm the biological function of SRD5A2 in hyp ..read more
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Human dental pulp stem cells differentiation into odontoblast and osteoblast-like cells on scaffolds contain bioactive materials
Cellular and Molecular Biology
by Ganjo Ghawtha Khudhur, Diyar Khalid Bakr
1M ago
Epigenetic change has been found to play an important role in cell differentiation and regulation and the dental pulp stem cell in tissue engineering is gaining attention due to the ability of cells to differentiate into odontoblast and other cells. This study evaluated the influence of poly L- lactic acid with hydroxyapatite-coated with polyaniline scaffold (PLLA/HA/PANI) on dental pulp stem cell (DPSC) proliferation and differentiation. After scaffold preparation and DPSCs seeding, the cells proliferation and differentiation were evaluated by immunocytochemistry assay and cell viability was ..read more
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Efficacy of ozonated olive oil against peri-implant microbes isolated from peri-implantitis
Cellular and Molecular Biology
by Ranj Nadhim Salaie, Shehab Ahmed Hamad, Zhala Dara Meran
1M ago
This study aimed to investigate the antibacterial and antimicrobial activity of ozone gel against oral biofilms grown on titanium dental implant discs. The experiment used medical grade five titanium discs on which peri-implant isolated biofilms were grown. The experimental groups were control, Streptococcus mutans (S. mutans) and Granulicatella adiacens (G. adiacens), (n = 6). The oral microbes grown on titanium discs were exposed to ozone gel for 3 minutes and the antibacterial activity was assessed by turbidity test and adherence test for the antibiofilm activity test. Bacterial morphology ..read more
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Effect of bone-marrow-derived mesenchymal stem cells on the rate of orthodontic tooth movements in rabbits
Cellular and Molecular Biology
by Rawand Jasim Othman, Omar Fawzi Chawshli
1M ago
Mesenchymal stem cells from bone marrow, such as bone marrow aspirate concentrate (BMAC) and cultured and isolated bone marrow mesenchymal stem cells (BM-MSCs), have been used as therapeutic alternatives to enhance remodeling in the bone. Objective: This study aimed to evaluate the effects of BMAC and BM-MSCs on orthodontic tooth movements in rabbits. Methods: A100- gram nickel–titanium closed-coil springs were used to initiate orthodontic tooth movement of the lower first premolars in 35 male New Zealand rabbits for 21 days. Using a split-mouth design, autologous BMAC or BM-MSCs were submucos ..read more
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Nur77 induced by HIF-1α mediates vascular remodeling in hypoxic pulmonary hypertension
Cellular and Molecular Biology
by Congzheng Mao, Jian Huang, Haichao Liu, Yi Liu, Zhenhong Hu, Ruijuan Xu
1M ago
Nur77 is a member of the NR4A subfamily of orphan nuclear receptors that is expressed and has a function within the immune system. This study aimed to investigate the role of Nur77 in hypoxic pulmonary hypertension. SPF male SD rats were exposed in hypobaric chamber simulating 5000 m high altitude for 0, 3, 7, 14, 21 or 28 days. Rat pulmonary artery smooth muscle cells (RPASMCs) were cultured under normoxic conditions (5% CO2-95% ambient air) or hypoxic conditions (5% O2 for 6 h, 12 h, 24 h, 48 h). Hypoxic rats developed pulmonary arterial remodeling and right ventricular hypertrophy with sign ..read more
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Exploration of mitochondrial autophagy related genes in the diagnosis model construction and molecular marker mining of Alzheimer's disease based on multi-omics integration
Cellular and Molecular Biology
by Shan Wang, Jiejie Zhang, Haitao Zhang, Yihan Yang, Ya Wen
1M ago
Key features of Alzheimer's disease include neuronal loss, accumulation of beta-amyloid plaques, and formation of neurofibrillary tangles. These changes are due in part to abnormal protein metabolism, particularly the accumulation of amyloid beta. Mitochondria are the energy production centers within cells and are also the main source of oxidative stress. In AD, mitochondrial function is impaired, leading to increased oxidative stress and the production of more reactive oxidative substances, further damaging cells. Mitophagy is an important mechanism for maintaining mitochondrial health, helpi ..read more
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