Hi all. I'm quite frustrated with my thesis at the moment as my supervisor keeps telling me I need to add the data from my plaque assays. But I have. I have included pictures (not too much) labeled them, calculated the PFU/ml, and wrote about 80-100 words on each section. I'm confused about what more she wants? The reasoning and problems and similar study findings have gone in my discussion section so I have no idea what she actually means. I would appreciate any help ?? submitted by /u/Tiny-Zone705 [visit reddit] [comments ..read more

I know this will probably get deleted or reported and I'm sorry but I've been kind of spiraling on here over the last few days because of H5N1 and all this speculation in the news and on that r/H5N1_AvianFlu subreddit which reminds me of r/Coronavirus especially during the early parts of 2020. ​ Not to sound melodramatic but with H5N1 do we need to head for the hills again like its 2020? Professor Vincent Racaniello doesn't seem convinced but others seem pretty worried right now. submitted by /u/EdHuRus [visit reddit] [comments ..read more

Hi everyone, does anyone know the best online video lectures specifically for Medical/Clinical Virology, the kind taught in Med school. Now before you go off the rail with Racaniello's Virology lectures, they are much more virobiology than Clinical virology, I have also seen lecturio, osmosis and sketchy but I need something really detailed on a graduate level that goes in to the molecular mechanisms of the disease causes and the according pathophysiology. Thank you! submitted by /u/Additional_Region_64 [visit reddit] [comments ..read more

I need to create a viral culture, I intended on incubating some cells in FBS and infecting it with my virus, then incubate for a few hours at 36-37C. And then storing the culture at -20C, or perhaps dilute it first in some glycerin before storing at -20C. I know it would be ideal to store the virus at a temperature like -80C but currently my lab can only provide -20C. Can anyone provide me with any insight in how I can store the cultured virus healthy for as long as possible? Should I dilute the FBS culture with glycerol? Maybe with something else? Should I try separating the virus with a cen ..read more

The BV-BRC is tracking the H5N1 spread very closely, and we want to remind researchers we can help ease sequence submission to Genbank- our team is ready to help make this genomic information accessible - check out our sequence submission tool here. https://www.bv-brc.org/app/SequenceSubmission submitted by /u/bv-brc [visit reddit] [comments ..read more

submitted by /u/ZergAreGMO [visit reddit] [comments ..read more

It is standard practice to obtain an HA titer of 8 with an influenza virus sample before conducting the Hemagglutination Inhibition Assay. Everytime i have raised this doubt, I get answers to the tune of "Well its just how it is" I am turning to reddit for some sound reasoning. Please help a budding virologist out! submitted by /u/notcallipygian [visit reddit] [comments ..read more

Both types can infect animal and human hosts and undergo recombination? I’m curious why Influenza B cannot undergo antigenic shift like A can and acquire new antigen genes following co infection with another virus submitted by /u/bluish1997 [visit reddit] [comments ..read more

I am terrified about H5N1. Like really terrified. submitted by /u/Class_of_22 [visit reddit] [comments ..read more

submitted by /u/Class_of_22 [visit reddit] [comments ..read more