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CRISPRi–TnSeq maps genome-wide interactions between essential and non-essential genes in bacteria

Nature » Microbiology

by Bimal Jana, Xue Liu, Julien Dénéréaz, Hongshik Park, Dmitry Leshchiner, Bruce Liu, Clément Gallay, Junhao Zhu, Jan-Willem Veening, Tim van Opijnen

1w ago

Nature Microbiology, Published online: 19 July 2024; doi:10.1038/s41564-024-01759-x CRISPRi knockdown of essential genes combined with TnSeq of non-essential genes yields a high-throughput method for bacterial genetic interaction mapping ..read more

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MHC class II proteins mediate sialic acid independent entry of human and avian H2N2 influenza A viruses

Nature » Microbiology

by Umut Karakus, Milagros Sempere Borau, Patricia Martínez-Barragán, Josephine von Kempis, Soner Yildiz, Laura M. Arroyo-Fernández, Marie O. Pohl, Julia A. Steiger, Irina Glas, Annika Hunziker, Adolfo García-Sastre, Silke Stertz

1w ago

Nature Microbiology, Published online: 15 July 2024; doi:10.1038/s41564-024-01771-1 MHC class II proteins from humans, pigs, ducks, swans and chickens can serve as alternative receptor for virus entry of H2N2 influenza A viruses. This trait is conserved in Eurasian avian H2 IAV ..read more

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Bacterial reprogramming of tick metabolism impacts vector fitness and susceptibility to infection

Nature » Microbiology

by Sourabh Samaddar, Agustin Rolandelli, Anya J. O’Neal, Hanna J. Laukaitis-Yousey, Liron Marnin, Nisha Singh, Xiaowei Wang, L. Rainer Butler, Parisa Rangghran, Chrysoula Kitsou, Francy E. Cabrera Paz, Luisa Valencia, Camila R. Ferraz, Ulrike G. Munderloh, Benedict Khoo, Benjamin Cull, Kristin L. Rosche, Dana K. Shaw, Jonathan Oliver, Sukanya Narasimhan, Erol Fikrig, Utpal Pal, Gary M. Fiskum, Brian M. Polster, Joao H. F. Pedra

1w ago

Nature Microbiology, Published online: 12 July 2024; doi:10.1038/s41564-024-01756-0 Bacteria reprogram tick metabolism impacting vector fitness and susceptibility to bacterial infection ..read more

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A safe, effective and adaptable live-attenuated SARS-CoV-2 vaccine to reduce disease and transmission using one-to-stop genome modifications

Nature » Microbiology

by Jacob Schön, G. Tuba Barut, Bettina Salome Trüeb, Nico Joel Halwe, Inês Berenguer Veiga, Annika Kratzel, Lorenz Ulrich, Jenna N. Kelly, Melanie Brügger, Claudia Wylezich, Adriano Taddeo, Etori Aguiar Moreira, Demeter Túrós, Llorenç Grau-Roma, Ann Kathrin Ahrens, Kore Schlottau, Tobias Britzke, Angele Breithaupt, Björn Corleis, Jana Kochmann, Blandina I. Oliveira Esteves, Lea Almeida, Lisa Thomann, Christelle Devisme, Hanspeter Stalder, Silvio Steiner, Sarah Ochsenbein, Kimberly Schmied, Fabien Labroussaa, Jörg Jores, Philip V’kovski, Vladimir Cmiljanovic, Marco P. Alves, Charaf Benarafa, Nadine Ebert, Donata Hoffmann, Martin Beer, Volker Thiel

1w ago

Nature Microbiology, Published online: 12 July 2024; doi:10.1038/s41564-024-01755-1 A live-attenuated SARS-CoV-2 vaccine based on one-to-stop codon modifications can elicit broad, systemic and mucosal immunity, as well as reduced transmission of SARS-CoV-2 in vivo ..read more

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A dynamic subpopulation of CRISPR–Cas overexpressers allows Streptococcus pyogenes to rapidly respond to phage

Nature » Microbiology

by Marie J. Stoltzfus, Rachael E. Workman, Nicholas C. Keith, Joshua W. Modell

1w ago

Nature Microbiology, Published online: 12 July 2024; doi:10.1038/s41564-024-01748-0 Subpopulations of Streptococcus pyogenes CRISPR mutants with overactive CRISPR–Cas activity wax and wane in response to phage infection pressure, enabling phage evasion without requiring a transient response in any one cell ..read more

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Multikingdom and functional gut microbiota markers for autism spectrum disorder

Nature » Microbiology

by Qi Su, Oscar W. H. Wong, Wenqi Lu, Yating Wan, Lin Zhang, Wenye Xu, Moses K. T. Li, Chengyu Liu, Chun Pan Cheung, Jessica Y. L. Ching, Pui Kuan Cheong, Ting Fan Leung, Sandra Chan, Patrick Leung, Francis K. L. Chan, Siew C. Ng

1w ago

Nature Microbiology, Published online: 08 July 2024; doi:10.1038/s41564-024-01739-1 Faecal metagenomes and machine learning reveal a panel of 31 multikingdom microbiome markers and microbiome functions that are associated with autism spectrum disorder in male and female children ..read more

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Environmentally dependent interactions shape patterns in gene content across natural microbiomes

Nature » Microbiology

by Kyle Crocker, Kiseok Keith Lee, Milena Chakraverti-Wuerthwein, Zeqian Li, Mikhail Tikhonov, Madhav Mani, Karna Gowda, Seppe Kuehn

2w ago

Nature Microbiology, Published online: 08 July 2024; doi:10.1038/s41564-024-01752-4 Global soil sequencing data and enrichment experiments show trade-offs in denitrification phenotypes driven by pH, underscoring how the environment shapes ecological interactions and gene content ..read more

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CRISPR-based functional profiling of the Toxoplasma gondii genome during acute murine infection

Nature » Microbiology

by Christopher J. Giuliano, Kenneth J. Wei, Faye M. Harling, Benjamin S. Waldman, Madeline A. Farringer, Elizabeth A. Boydston, Tammy C. T. Lan, Raina W. Thomas, Alice L. Herneisen, Allen G. Sanderlin, Isabelle Coppens, Jeffrey D. Dvorin, Sebastian Lourido

2w ago

Nature Microbiology, Published online: 08 July 2024; doi:10.1038/s41564-024-01754-2 A genome-wide CRISPR-based screen of Toxoplasma gondii during mouse infection reveals previously uncharacterized factors contributing to parasite virulence ..read more

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Algal methylated compounds shorten the lag phase of Phaeobacter inhibens bacteria

Nature » Microbiology

by Martin Sperfeld, Delia A. Narváez-Barragán, Sergey Malitsky, Veronica Frydman, Lilach Yuda, Jorge Rocha, Einat Segev

3w ago

Nature Microbiology, Published online: 05 July 2024; doi:10.1038/s41564-024-01742-6 Algal-associated bacteria assimilate methyl groups through the methionine cycle as ready-made carbon building blocks that jump-start growth ..read more

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Enterococcus faecalis-derived adenine enhances enterohaemorrhagic Escherichia coli Type 3 Secretion System-dependent virulence

Nature » Microbiology

by Fernando H. Martins, Thibaut Rosay, Anubama Rajan, Hannah E. Carter, Tayah Turocy, Andres Mejia, Jason M. Crawford, Anthony W. Maresso, Vanessa Sperandio

3w ago

Nature Microbiology, Published online: 04 July 2024; doi:10.1038/s41564-024-01747-1 Enterococcus faecalis-derived adenine increases enterohaemorrhagic Escherichia coli virulence by relieving Hha-dependent repression of Type 3 Secretion System activity ..read more

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