If you're just wondering if the guys in suits who say words like "depreciation" and "gestation lag" (and might seem like the sort of guys having fun in college while you were doing enzyme kinetic curves) are just making stuff up - they do appear to have some math to rely on here and there. This comes from what might be the worst name of an academic journal I've ever heard of, but it's funny how much (what I can understand of it) seems to line up with reality. That Orbitrap Velos didn't get more valuable while you were using it. It likely got less. And how long was it before you felt ..read more

As part of a big review we've been working on for a while, and might be a little late (sorry) we've been really digging into the data behind "single cell proteomics" studies. I really really like this review from friends in Austria because I don't know how to do these publication search term analyses and I can just steal theirs. To people in the outside world it probably looks like we've completed hundreds - or at least....dozens.... of studies where single normalish-sized single cells were analyzed by LCMS.  Our list does not have even 2 dozen actually studies. I'm sure that will cha ..read more

This is a thoughtful addition to the challenges with carrier channel boosting proteomics samples. The isotopic impurities are something you can largely ignore when your signal is roughly equivalent, but when it isn't, it's a big problem. It does make you wonder if there might be a way to make expensive multiplexing tags that are...I dunno.... actually pure...?  ..read more

  Ready to get super pumped about leaving the lab and not thinking about proteomics for a few days? I'll help you get started with  QUAAAAAALLLLIIIIIIIITTTTTYYYYYY  COOOOONNNTRRRROOOOOLL PREEEPRIINNNTTS! Let's start with this great new Primer!  Just like how you have to put primer on right after you sand most things and before you do the actual hard part of applying the paint people will see --- this'll get you ready for the this bit of important misery ..read more

  I'm re-reading this paper from last spring and - wow -  Don't want to read? Just interested in how a bunch of relevant drugs alter the proteome, phosphoproteome, ubiquitinome or acetylome of different cell lines?  Check out this ridiculously nice data portal here!  ..read more

  I had another mandatory Microsoft update thing which 1) enabled their ....sub-performing.... blend of ChatGPT and Bing in the lower right corner of my screen where the button should be to get me back to my desktop... and  2) Makes it so that if I open a document from my desktop and go to save it as a new name it defaults to some imaginary "cloud" thing so I will never ever be able to find it again. You can "other save options" or you can: Open every one of the MS Office things you use  Go to file Go allllllllllllllllllllllllllllllllllllll the way down to Options Go to Save ..read more

  So...front end ion mobility things like TIMS are gas vs electric pull, right? So what happens if you run 5x the flow rate for your LC input? Will that alter the gas pressure enough to change your perception of the collisional cross section (CCS) and thereby change the observed 1/k0 values?  Really really cool study on this here!  You'll note they're using the ESI source, but if you're using the CaptiveSpray you're blasting your solvent directly into your glass capillary, right? I suspect that you'll see something similar when going from 100nL to 2 uL/min!  ..read more

  You know when you finally solve a mystery that is driving you completely bonkers and you get that relief at first that you solved it. But then you realize how many samples you have to go back and rerun and you think "maybe I should really truly quit being a mass spectrometrist"? I'm having one of those days.  Quick solution. If you upgrade your nice TIMSTOF instrument to TIMSControl 5.0 you'll get the thing above that I labeled #1 and - it is AWESOME. You get much better control over your mobilogram windows that can be guided by your real data. Using it right for DDA can give you ..read more

Josip Blonder had one of the biggest effects on my development as a scientist in this field. He's got a fancy emeritus/pseudo-retired status at the NIH now, but apparently he's not just fishing off of Hvar, even if he isn't pulling down the surfaceome these days. He's updated Proteomics for Drug Discovery and it will be out in August! And when I stumbled on this, I also found this one is coming out just before it!  The first book on single cell proteomics by mass spectrometry!  This isn't like preodering a video game. The corporate shmucks can't just cut development and bug testin ..read more

  How cool does that sound? Zoom should be good for a huge number of external participants! You can check it out here if you're interested. https://jhjhm.zoom.us/j/99656763944 ..read more